Kocan K M, Wickwire K B, Ewing S A, Hair J A, Barron S J
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
Am J Vet Res. 1988 Jul;49(7):1010-3.
On each day of feeding on susceptible calves, salivary glands obtained from groups of adult ticks that transmitted Anaplasma marginale were examined for A marginale colonies by use of light microscopy and transmission electron microscopy. On day 8 of feeding, salivary glands were examined, using fluorescein-labeled antibody and methyl green-pyronine stain. Use of fluorescein-labeled antibody consistently revealed small numbers of fluorescent foci in salivary gland acinar cells obtained from ticks that had fed for 8 days. Colonies of A marginale were seen by transmission electron microscopy only in salivary gland acini of male ticks; these colonies could not be identified, using light microscopy, in companion 1-micron plastic sections stained with Mallory stain. Methyl green-pyronine stain, used commonly to detect theilerial parasites in tick salivary glands, did not differentiate A marginale from cytoplasmic inclusions normally found in salivary gland acinar cells.
在以易感小牛为食的每一天，从传播边缘无浆体的成年蜱虫群体中获取唾液腺，通过光学显微镜和透射电子显微镜检查边缘无浆体菌落。在进食第8天，使用荧光素标记抗体和甲基绿-派洛宁染色法检查唾液腺。使用荧光素标记抗体始终在进食8天的蜱虫的唾液腺腺泡细胞中发现少量荧光灶。仅在雄蜱的唾液腺腺泡中通过透射电子显微镜观察到边缘无浆体菌落；在经马洛里染色的1微米塑料切片中，用光学显微镜无法识别这些菌落在对照中的情况。常用于检测蜱唾液腺中泰勒原虫寄生虫的甲基绿-派洛宁染色法，无法区分边缘无浆体与唾液腺腺泡细胞中通常存在的细胞质内含物。
