Kocan K M, Teel K D, Hair J A
Am J Vet Res. 1980 Feb;41(2):183-6.
Cattle were inoculated with a Virginia isolate of Anaplasma marginale Theiler and served as an infective source for laboratory-reared Dermacentor andersoni Stiles and D variabilis (Say) nymphs. Transstadial transmission of A marginale was demonstrated by feeding the newly molted adult ticks of both species on susceptible cattle and by inoculation of gut and salivary gland homogenates collected from adult ticks at postattachment days 5 and 7. Oral secretions collected from the same group of ticks and inoculated into a susceptible cow did not cause infection. Fluorescent antibody studies of infected tick gut and salivary glands were positive, although smears of tick oral secretions were negative for fluorescence.
用弗吉尼亚州的边缘无形体泰勒分离株对牛进行接种，并将其作为实验室饲养的安氏革蜱和变异革蜱若虫的感染源。通过让这两种新蜕皮的成年蜱吸食易感牛，并接种在附着后第5天和第7天从成年蜱收集的肠道和唾液腺匀浆，证明了边缘无形体的经变态传播。从同一组蜱收集的口腔分泌物接种到易感母牛体内未引起感染。对感染蜱的肠道和唾液腺进行荧光抗体研究呈阳性，尽管蜱口腔分泌物涂片的荧光检测为阴性。
