Collins J K, Jensen R, Smith G H, Flack D E, Kerschen R, Bennett B W, Jones R L, Alexander A F
Diagnostic Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
Am J Vet Res. 1988 Jul;49(7):1045-9.
Thirty-three cattle with fatal respiratory tract disease were examined for gross and histologic lesions and for the presence of viral and bacterial agents in the lungs. Fifteen cattle had lesions characteristic of atypical interstitial pneumonia (AIP), and 18 had other respiratory tract diseases, including infectious bovine rhinotracheitis, shipping fever pneumonia, bronchopneumonia, pulmonary abscess, and edema of the trachea. Gross necropsy findings in the cattle with AIP were uncollapsed and emphysematous lungs; histopathologic findings included interstitial edema, thickening of alveolar walls, hyaline membrane formation, and hyperplasia of type-II pneumonocytes. The infective agents found in the lungs of the 33 cattle included bovine respiratory syncytial virus, bovine herpesvirus type 1, Pasteurella sp, mycoplasmas, and Corynebacterium pyogenes. Bovine respiratory syncytial virus was detected by use of immunofluorescence and immunoperoxidase on lung tissue sections; bovine herpesvirus type 1 was detected by these techniques and by isolation of the virus. Bovine respiratory syncytial virus was significantly (P = 0.01) associated with lesions of AIP (11 of 15), compared with those of other respiratory tract diseases (5 of 18).
对33头患有致命呼吸道疾病的牛进行了大体和组织学病变检查，并检测了肺中病毒和细菌病原体的存在情况。15头牛有非典型间质性肺炎（AIP）的特征性病变，18头牛患有其他呼吸道疾病，包括传染性牛鼻气管炎、运输热肺炎、支气管肺炎、肺脓肿和气管水肿。患有AIP的牛的大体尸检结果为肺未塌陷且气肿；组织病理学结果包括间质水肿、肺泡壁增厚、透明膜形成和II型肺细胞增生。在33头牛的肺中发现的感染因子包括牛呼吸道合胞病毒、1型牛疱疹病毒、巴斯德氏菌属、支原体和化脓棒状杆菌。通过对肺组织切片使用免疫荧光和免疫过氧化物酶检测牛呼吸道合胞病毒；通过这些技术以及病毒分离检测1型牛疱疹病毒。与其他呼吸道疾病（18头中的5头）相比，牛呼吸道合胞病毒与AIP病变（15头中的11头）显著相关（P = 0.01）。
