Chuang L Y, Chang C C
Department of Biochemistry, Kaohsiung Medical College, Taiwan, Republic of China.
Biochem Biophys Res Commun. 1988 Sep 15;155(2):870-6. doi: 10.1016/s0006-291x(88)80576-x.
alpha-Bungarotoxin was digested with acid protease A and the resulting peptide fragments were isolated by reverse-phase HPLC. The antigenic activity of the peptide fragments were examined by competitive solid phase radioimmunoassay and the probably sequences were assigned. The two antigenic regions are likely located around the residues 34-41 and 68-74 of alpha-bungarotoxin, and the other two may be within the residues 3-26 and 45-67, respectively. Radioimmunoassay revealed a maximum number of four antigenic determinants of alpha-bungarotoxin that can simultaneously be occupied by antibody.
用酸性蛋白酶A消化α-银环蛇毒素，所得肽片段通过反相高效液相色谱法分离。通过竞争性固相放射免疫测定法检测肽片段的抗原活性，并确定可能的序列。两个抗原区域可能位于α-银环蛇毒素的34-41位和68-74位残基附近，另外两个可能分别位于3-26位和45-67位残基内。放射免疫测定显示，α-银环蛇毒素最多有四个抗原决定簇可同时被抗体占据。
