抗α-银环蛇毒素的中和性单克隆抗体：抗体的制备与特性鉴定以及α-银环蛇毒素抗原区域的定位

Neutralizing monoclonal antibody specific for alpha-bungarotoxin: preparation and characterization of the antibody, and localization of antigenic region of alpha-bungarotoxin.

作者信息

Kase R, Kitagawa H, Hayashi K, Tanoue K, Inagaki F

机构信息

Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, Japan.

出版信息

FEBS Lett. 1989 Aug 28;254(1-2):106-10. doi: 10.1016/0014-5793(89)81018-x.

DOI:10.1016/0014-5793(89)81018-x

PMID:2476330

Abstract

We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-bungarotoxin. The antigenic determinant was located on the peptide fragment containing S34-S35-R36-G37-K38, which forms a part of the expected site that binds to the acetylcholine receptor proteins.

摘要

我们制备了一种能在体内中和毒素生物活性的抗α-银环蛇毒素特异性单克隆抗体。基于使用其他三种长神经毒素和α-银环蛇毒素肽片段的交叉反应实验，确定了与该抗体特异性结合的抗原决定簇。抗原决定簇位于包含S34 - S35 - R36 - G37 - K38的肽片段上，该片段构成了预期与乙酰胆碱受体蛋白结合位点的一部分。