Samsami Dehaghani Alamtaj, Razmjoei Parisa, Parsanezhad Mohammad Ebrahim
Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, P.O. Box 71345-1798, Shiraz, Iran ; Department of Obstetrics and Gynecology, Shahid Faghihi Hospital, Zand Avenue, P.O. Box 7134-3119, Shiraz, Iran.
Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, P.O. Box 71345-1798, Shiraz, Iran.
J Obstet Gynaecol India. 2014 Feb;64(1):47-52. doi: 10.1007/s13224-013-0451-x. Epub 2013 Sep 25.
To determine the serum levels of anti-dsDNA, anti-histone, and anti-nucleosome antibodies after laparoscopic ovarian electrocauterization in patients with polycystic ovarian syndrome (PCOS).
Our study was performed on 35 patients with PCOS resistant to medical therapy, 35 patients with unexplained infertility, and 35 healthy fertile individuals. Patients with PCOS underwent laparoscopic electrocauterization while those with unexplained infertility underwent diagnostic laparoscopy. Serum levels of anti-dsDNA, anti-histone, and anti-nucleosome antibodies were measured at baseline and 1 month after operation and were compared between groups.
Baseline characteristics were similar between groups. Patients with PCOS had significantly higher levels of anti-dsDNA compared to unexplained infertility (p < 0.001) and control groups (p = 0.001). Anti-histone antibodies were higher in PCOS group compared to control group (p = 0.001). In those patients suffering from PCOS, anti-histone antibody increased significantly 1 month after ovarian electrocauterization (p = 0.017). Similarly, serum levels of anti-nucleosome antibodies increased significantly 1 month after operation (p < 0.001).
Laparoscopic ovarian electrocauterization in patients with PCOS results in increased levels of anti-histone and anti-nucleosome antibodies. Anti-dsDNA, anti-histone, and anti-nucleosome antibodies also increase after diagnostic laparoscopy in those with unexplained infertility. Patients with PCOS have higher levels of anti-dsDNA and anti-histone antibodies compared to those with unexplained infertility and healthy fertile subjects.
测定多囊卵巢综合征（PCOS）患者腹腔镜卵巢电灼术后抗双链DNA、抗组蛋白和抗核小体抗体的血清水平。
我们对35例药物治疗抵抗的PCOS患者、35例不明原因不孕症患者和35名健康可育个体进行了研究。PCOS患者接受腹腔镜电灼术，不明原因不孕症患者接受诊断性腹腔镜检查。在基线和术后1个月测量抗双链DNA、抗组蛋白和抗核小体抗体的血清水平，并在组间进行比较。
各组基线特征相似。与不明原因不孕症患者（p<0.001）和对照组（p=0.001）相比，PCOS患者的抗双链DNA水平显著更高。与对照组相比，PCOS组的抗组蛋白抗体更高（p=0.001）。在患有PCOS的患者中，卵巢电灼术后1个月抗组蛋白抗体显著增加（p=0.017）。同样，术后1个月抗核小体抗体的血清水平显著增加（p<0.001）。
PCOS患者腹腔镜卵巢电灼术导致抗组蛋白和抗核小体抗体水平升高。不明原因不孕症患者诊断性腹腔镜检查后抗双链DNA、抗组蛋白和抗核小体抗体也会增加。与不明原因不孕症患者和健康可育受试者相比，PCOS患者的抗双链DNA和抗组蛋白抗体水平更高。
