D-002对大鼠实验性诱导胃食管反流的保护作用。
Protective effects of D-002 on experimentally induced gastroesophageal reflux in rats.
作者信息
Zamora Zullyt, Molina Vivian, Mas Rosa, Ravelo Yazmin, Perez Yohany, Oyarzabal Ambar
机构信息
Zullyt Zamora, Vivian Molina, Rosa Mas, Yazmin Ravelo, Yohany Perez, Ambar Oyarzabal, Department of Pharmacology, Centre of Natural Products, National Centre for Scientific Research, Havana 10600, Cuba.
出版信息
World J Gastroenterol. 2014 Feb 28;20(8):2085-90. doi: 10.3748/wjg.v20.i8.2085.
AIM
To investigate the effects of beeswax alcohols (D-002) on the esophageal damage induced by gastroesophageal reflux (GER) in rats.
METHODS
Sixty male rats were randomized into six groups (10 rats/group): a negative control and five groups with experimentally induced GER: a positive vehicle control, three treated with D-002 (25, 100 and 200 mg/kg, respectively), and one with omeprazole 10 mg/kg. All treatments were given by gastric gavage. One hour after dosing, GER was produced by simultaneous ligation of the pyloric end and the forestomach. Esophageal lesions index (ELI), gastric secretion volume and acidity, and esophageal malondialdehyde (MDA) and sulfhydryl (SH) group concentrations were measured. Statistical significance was considered at P < 0.05.
RESULTS
As compared to the negative control, the positive control group exhibited increased ELI (5.2 ± 0.33 vs 0 ± 0, P = 0.0003), gastric secretion volume (2.69 ± 0.09 vs 0.1 ± 0.0, P = 0.0003) and acidity (238 ± 19.37 vs 120.0 ± 5.77, P = 0.001), and esophageal concentrations of MDA (2.56 ± 0.1 vs 1.76 ± 0.28, P = 0.001) and SH groups (1.02 ± 0.05 vs 0.56 ± 0.08, P = 0.0003). D-002 (25, 100 and 200 mg/kg) reduced ELI (3.36 ± 0.31, 2.90 ± 0.46 and 2.8 ± 0.23, respectively) vs the positive control (5.2 ± 0.33) (P = 0.004; P = 0.002; P = 0.001, respectively). There were no significant changes in acidity with D-002 treatment, and only the highest dose reduced the volume of the gastric secretion (1.92 ± 0.25) vs the positive control (2.69 ± 0.09, P = 0.013). D-002 (25, 100 and 200 mg/kg) lowered the esophageal MDA (2.05 ± 0.16, 1.98 ± 0.22 and 1.93 ± 0.22, respectively) (P = 0.01; P = 0.03; P = 0.03, respectively) and SH group concentration (0.87 ± 0.06, 0.79 ± 0.08 and 0.77 ± 0.06, respectively) (P = 0.04; P = 0.04; P = 0.02) vs the positive control (2.56 ± 0.10 and 1.02 ± 0.05, respectively). Omeprazole decreased ELI (2.54 ± 0.47), gastric secretion volume (1.97 ± 0.14) and acidity (158.5 ± 22.79), esophageal MDA (1.87 ± 0.13) and SH group (0.72 ± 0.05) concentrations vs the positive control (P = 0.002; P = 0.001; P = 0.02; P = 0.003; P = 0.002, respectively).
CONCLUSION
Acute oral administration of D-002 decreased macroscopic esophageal lesions and oxidative stress in rats with experimentally induced GER, without modifying gastric secretion acidity.
目的
研究蜂蜡醇（D - 002）对实验性诱导的大鼠胃食管反流（GER）所致食管损伤的影响。
方法
将60只雄性大鼠随机分为6组（每组10只）：一个阴性对照组和5个实验性诱导GER的组：一个阳性赋形剂对照组、3个分别用D - 002（25、100和200 mg/kg）处理的组以及一个用奥美拉唑10 mg/kg处理的组。所有处理均通过胃内灌胃给予。给药1小时后，通过同时结扎幽门端和前胃来诱导GER。测量食管损伤指数（ELI）、胃分泌量和酸度以及食管丙二醛（MDA）和巯基（SH）基团浓度。P < 0.05时认为具有统计学意义。
结果
与阴性对照组相比，阳性对照组的ELI升高（5.2 ± 0.33对0 ± 0，P = 0.0003）、胃分泌量升高（2.69 ± 0.09对0.1 ± 0.0，P = 0.0003）和酸度升高（238 ± 19.37对120.0 ± 5.77，P = 0.001），以及食管MDA浓度升高（2.56 ± 0.1对1.76 ± 0.28，P = 0.001）和SH基团浓度升高（1.02 ± 0.05对0.56 ± 0.08，P = 0.0003）。与阳性对照组（5.2 ± 0.33）相比，D - 002（25、100和200 mg/kg）降低了ELI（分别为3.36 ± 0.31、2.90 ± 0.46和2.8 ± 0.23）（分别为P = 0.004；P = 0.002；P = 0.001）。D - 002处理后酸度无显著变化，仅最高剂量降低了胃分泌量（1.92 ± 0.25），与阳性对照组（2.69 ± 0.09）相比（P = 0.013）。与阳性对照组（分别为2.56 ± 0.10和1.02 ± 0.05）相比，D - 002（25、100和200 mg/kg）降低了食管MDA浓度（分别为2.05 ± 0.16、1.98 ± 0.22和1.93 ± 0.22）（分别为P = 0.01；P = 0.03；P = 0.03）和SH基团浓度（分别为0.87 ± 0.06、0.79 ± 0.08和0.77 ± 0.06）（分别为P = 0.
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