Arabaci Gulnur, Usluoglu Ayse
Department of Chemistry, Faculty of Science and Arts, Sakarya University, Serdivan, 54187 Sakarya, Turkey.
ScientificWorldJournal. 2014 Jan 21;2014:685975. doi: 10.1155/2014/685975. eCollection 2014.
Water pollution due to release of industrial wastewater has already become a serious problem in almost every industry using dyes to color its products. In this work, polyphenol oxidase enzyme from quince (Cydonia Oblonga) leaves immobilized on calcium alginate beads was used for the successful and effective decolorization of textile industrial effluent. Polyphenol oxidase (PPO) enzyme was extracted from quince (Cydonia Oblonga) leaves and immobilized on calcium alginate beads. The kinetic properties of free and immobilized PPO were determined. Quince leaf PPO enzyme stability was increased after immobilization. The immobilized and free enzymes were employed for the decolorization of textile dyes. The dye solutions were prepared in the concentration of 100 mg/L in distilled water and incubated with free and immobilized quince (Cydonia Oblonga) leaf PPO for one hour. The percent decolorization was calculated by taking untreated dye solution. Immobilized PPO was significantly more effective in decolorizing the dyes as compared to free enzyme. Our results showed that the immobilized quince leaf PPO enzyme could be efficiently used for the removal of synthetic dyes from industrial effluents.
工业废水排放导致的水污染在几乎每个使用染料给产品染色的行业中都已成为一个严重问题。在这项工作中，固定在海藻酸钙珠上的温柏（Cydonia Oblonga）叶中的多酚氧化酶被用于成功且有效地使纺织工业废水脱色。从温柏（Cydonia Oblonga）叶中提取多酚氧化酶（PPO）并将其固定在海藻酸钙珠上。测定了游离和固定化PPO的动力学性质。固定化后温柏叶PPO酶的稳定性提高。将固定化和游离酶用于纺织染料的脱色。染料溶液在蒸馏水中以100 mg/L的浓度配制，并与游离和固定化的温柏（Cydonia Oblonga）叶PPO孵育一小时。通过使用未处理的染料溶液计算脱色百分比。与游离酶相比，固定化PPO在使染料脱色方面明显更有效。我们的结果表明，固定化的温柏叶PPO酶可有效地用于去除工业废水中的合成染料。
