Zhou Fang, Lin Haiyan, Luo Yong, Liu Changwei
School of Chemistry and Environmental Sciences, Xiangnan University, Chenzhou 423000, China.
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
Plants (Basel). 2025 Jul 28;14(15):2335. doi: 10.3390/plants14152335.
Polyphenol oxidase (PPO) is a metalloproteinase widely present in plant organelles that plays crucial roles in photosynthesis, pest and disease resistance, growth and development, and flower color formation. Due to the high cost and reuse difficulties of plant PPO in applications, immobilization has emerged as a key technology to improve its stability, recyclability, and reusability. Immobilized plant PPO has been widely used in environmental and detection fields. This review examines different immobilization methods and carrier materials for plant PPO and summarizes its applications in wastewater treatment, biosensor detection, food preservation, and theaflavin synthesis. Finally, current challenges and future opportunities for immobilized plant PPO are discussed.
多酚氧化酶（PPO）是一种广泛存在于植物细胞器中的金属蛋白酶，在光合作用、病虫害抗性、生长发育以及花色形成中发挥着关键作用。由于植物PPO在应用中成本高昂且难以重复利用，固定化技术已成为提高其稳定性、可回收性和再利用性的关键技术。固定化植物PPO已广泛应用于环境和检测领域。本文综述了植物PPO的不同固定化方法和载体材料，并总结了其在废水处理、生物传感器检测、食品保鲜和茶黄素合成中的应用。最后，讨论了固定化植物PPO目前面临的挑战和未来的机遇。
