Maikai Victor Ambrose, Maikai Beatty Viv, Kobo Patricia Ishyaku
College of Agriculture and Animal Science, Ahmadu Bello University, P.M.B. 2134, Mando, Kaduna, Nigeria.
Department of Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
J Parasitol Res. 2014;2014:904318. doi: 10.1155/2014/904318. Epub 2014 Jan 22.
Trypanosomosis is a debilitating disease affecting mainly livestock and humans in tropical Africa. Chemically synthesized drugs and medicinal plants have been used in the treatment and control of this disease. In this study, the in vitro effect of aqueous extracts and fraction IV extract of Ximenia americana stem bark on Trypanosoma congolense DNA was investigated. The extracts were incubated with the parasites in vitro at 300 mg/mL aqueous extract and 25 mg/mL fraction IV portion for 30, 60, and 120 mins. The DNA of the trypanosomes was isolated and digested using ECOR1 enzyme and subsequently PCR was carried out. Results showed that aqueous extract and fraction IV portion immobilized 55% and 90% of the trypanosomes after 30-minute incubation. Subsequent isolation of the parasite DNA and agarose gel electrophoresis did not reveal that cell death was as a result of DNA fragmentation. This suggests that cell death was by another mechanism of action.
锥虫病是一种使人虚弱的疾病，主要影响热带非洲的家畜和人类。化学合成药物和药用植物已被用于治疗和控制这种疾病。在本研究中，研究了铁青树茎皮水提取物和IV部分提取物对刚果锥虫DNA的体外作用。将提取物与寄生虫在体外以300mg/mL水提取物和25mg/mL IV部分在30、60和120分钟进行孵育。分离锥虫的DNA，用ECOR1酶消化，随后进行PCR。结果表明，孵育30分钟后，水提取物和IV部分分别使55%和90%的锥虫固定。随后对寄生虫DNA的分离和琼脂糖凝胶电泳未显示细胞死亡是由DNA片段化导致的。这表明细胞死亡是通过另一种作用机制。
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