Rossi Gabriele, Mangiagalli Gerard, Paracchini Giulia, Paltrinieri Saverio
Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy.
Vet Clin Pathol. 2014 Mar;43(1):6-14. doi: 10.1111/vcp.12121.
Information about laboratory reference intervals (RIs) of European Hedgehog (Erinaceus europaeus) hospitalized at rehabilitation centers is scarce.
The purpose of this study was to establish hematologic and biochemical RIs for rehabilitated hedgehogs before the release into the wild, and to assess whether sex and management of the center influence laboratory results.
Blood was collected from 50 hedgehogs at 3 centers. Thirty-eight animals were included in the study based on normal body weight, absence of clinical signs of disease, Bunnell index > 0.80, and absence of hibernation during overwintering. CBCs were performed using an automated laser cell counter followed by morphologic analysis of blood smears. Clinical biochemistry was performed using an automated spectrophotometer. RIs were determined as recommended by the ASVCP guidelines.
Hematology profiles revealed a prevalence of lymphocytes, a constant presence of nucleated RBCs, Howell-Jolly bodies and basophils, and bilobed nuclei in neutrophils and eosinophils. Biochemistry profiles were characterized by higher creatinine and urea concentrations, and higher ALP and GGT activities compared with other domestic species. The sex did not influence the results. Conversely, numbers of eosinophils, activated and large granular lymphocytes, and concentrations of total protein, glucose and cholesterol were different among the centers, likely due to different management practices (eg, antiparasitic treatments, environmental exposure to microorganisms, diet).
The RIs established in this study can be used to monitor the health status of hedgehogs in rehabilitation centers. As management practices appeared to influence some variables, it is recommended to standardize the management protocols to minimize their influence on laboratory data.
关于在康复中心住院的欧洲刺猬（ Erinaceus europaeus ）实验室参考区间（RIs）的信息匮乏。
本研究的目的是为放归野外前的康复刺猬建立血液学和生化参考区间，并评估性别和中心管理方式是否会影响实验室检测结果。
从3个中心的50只刺猬采集血液。基于体重正常、无疾病临床症状、邦内尔指数>0.80以及越冬期间无冬眠情况，38只动物被纳入研究。使用自动激光细胞计数器进行全血细胞计数（CBCs），随后对血涂片进行形态学分析。使用自动分光光度计进行临床生化检测。参考区间按照美国兽医临床病理学家协会（ASVCP）指南的建议确定。
血液学检查结果显示淋巴细胞占优势，有核红细胞、豪-焦小体和嗜碱性粒细胞持续存在，中性粒细胞和嗜酸性粒细胞有双叶核。与其他家养物种相比，生化检查结果的特征是肌酐和尿素浓度较高，碱性磷酸酶（ALP）和γ-谷氨酰转移酶（GGT）活性较高。性别不影响检测结果。相反，各中心之间嗜酸性粒细胞、活化和大颗粒淋巴细胞的数量以及总蛋白、葡萄糖和胆固醇的浓度不同，这可能是由于管理方式不同（例如，抗寄生虫治疗、环境微生物暴露、饮食）。
本研究建立的参考区间可用于监测康复中心刺猬的健康状况。由于管理方式似乎会影响一些变量，建议规范管理方案，以尽量减少其对实验室数据的影响。
