Xu Jianhong, Deng Peng, Showmaker Kurt C, Wang Hui, Baird Sonya M, Lu Shi-En
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture/Jiangsu Academy of Agricultural Sciences, Nanjing, China; Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.
FEMS Microbiol Lett. 2014 Apr;353(2):98-105. doi: 10.1111/1574-6968.12411. Epub 2014 Apr 11.
Strain JX22, exhibiting a broad range of antimicrobial activities to fungal pathogens, was isolated and classified as representing Pseudomonas kilonensis. In this study, the mutant JX22MT1 was obtained by the EZ-Tn5 transposon mutation and showed no antifungal activity against Fusarium oxysporum f. sp. lycopersici as compared with wild-type strain JX22. The pqqC gene was disrupted in the mutant. Antifungal activity at the wild-type level was restored from the mutant JX22MT1 with the introduction of the functional pqqC gene, which encodes pyrroloquinoline-quinone synthesis protein C. The results suggest that pqqC is essential for antifungal activity of P. kilonensis JX22 against F. oxysporum f. sp. lycopersici.
菌株JX22对真菌病原体具有广泛的抗菌活性，该菌株被分离出来并归类为基洛假单胞菌（Pseudomonas kilonensis）。在本研究中，通过EZ-Tn5转座子突变获得了突变体JX22MT1，与野生型菌株JX22相比，该突变体对番茄枯萎病菌（Fusarium oxysporum f. sp. lycopersici）没有抗真菌活性。突变体中的pqqC基因被破坏。通过导入编码吡咯喹啉醌合成蛋白C的功能性pqqC基因，突变体JX22MT1恢复了野生型水平的抗真菌活性。结果表明，pqqC对于基洛假单胞菌JX22对番茄枯萎病菌的抗真菌活性至关重要。
