Hu Liang Bin, Shi Zhi Qi, Zhang Ting, Yang Zhi Min
College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
FEMS Microbiol Lett. 2007 Jul;272(1):91-8. doi: 10.1111/j.1574-6968.2007.00743.x. Epub 2007 May 8.
Strain B-FS01, isolated from rape (Brassica napus) stem infected by Slerotinia sclerotiorum and identified as Bacillus subtilis, exhibited predominantly antagonistic activities against Fusarium moniliforme Sheldon ATCC 38932. Antifungal active compounds (AAC) were isolated and purified from the cultures of strain B-FS01 against ATCC 38932. The HPLC/electron spray ionization/collision-induced dissociation mass spectrum of AAC revealed a cluster of fengycin homologues containing fengycins A, fengycins B and a new type of fengycin. Further toxic assay of AAC in vitro against F. moniliforme indicated that AAC could strongly inhibit the growth of both mycelia and spores. In addition, treatment with AAC significantly modified the maize seed infection by ATCC 38932.
从感染核盘菌的油菜（甘蓝型油菜）茎中分离得到的B-FS01菌株，经鉴定为枯草芽孢杆菌，对串珠镰刀菌Sheldon ATCC 38932表现出主要的拮抗活性。从B-FS01菌株针对ATCC 38932的培养物中分离并纯化了抗真菌活性化合物（AAC）。AAC的高效液相色谱/电喷雾电离/碰撞诱导解离质谱显示出一组包含丰原素A、丰原素B和一种新型丰原素的丰原素同系物。AAC对串珠镰刀菌的体外进一步毒性试验表明，AAC能强烈抑制菌丝体和孢子的生长。此外，用AAC处理显著改变了ATCC 38932对玉米种子的感染情况。
