经20年采样，斜纹夜蛾核型多角体病毒经口感染因子具有高度遗传稳定性。

High genetic stability of peroral infection factors from Anticarsia gemmatalis MNPV over 20years of sampling.

作者信息

Ferreira Briana C, Melo Fernando L, Souza Marlinda L, Moscardi Flávio, Báo Sônia N, Ribeiro Bergmann M

机构信息

Programa de Pós-Graduação em Patologia Molecular, Faculdade de Medicina, Campus Darcy Ribeiro, Universidade de Brasília (UnB), Asa Norte, 70910-900 Brasília, DF, Brazil; Embrapa Recursos Genéticos e Biotecnologia (Cenargen), Parque Estação Biológica, W5 Norte Final, 70770-917 Brasília, DF, Brazil.

Programa de Pós-Graduação em Patologia Molecular, Faculdade de Medicina, Campus Darcy Ribeiro, Universidade de Brasília (UnB), Asa Norte, 70910-900 Brasília, DF, Brazil.

出版信息

J Invertebr Pathol. 2014 May;118:66-70. doi: 10.1016/j.jip.2014.02.007. Epub 2014 Feb 28.

DOI:10.1016/j.jip.2014.02.007

PMID:24590109

Abstract

The Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) has been used as a biopesticide since the early 1980s in Brazil to control the major pest of soybean crops, the velvetbean caterpillar, Anticarsia gemmatalis. To monitor the genetic diversity over space and time we sequenced four pif genes (pif1, pif2, pif3 and pif4) from AgMNPV isolates collected from different regions of South America, as well as of seasonal isolates, sampled during a two-decade field experiment. Although all genes presented low levels of polymorphism, the pif-2 carries a slightly higher number of polymorphic sites. Overall, this study reveals that pif genes have remained stable after 20 years of repeated field application.

摘要

自20世纪80年代初以来，巴西一直使用豆夜蛾多核多角体病毒（AgMNPV）作为生物杀虫剂，以防治大豆作物的主要害虫——豆夜蛾（Anticarsia gemmatalis）。为了监测时空上的遗传多样性，我们对从南美洲不同地区收集的AgMNPV分离株以及在一项为期二十年的田间试验中采集的季节性分离株的四个pif基因（pif1、pif2、pif3和pif4）进行了测序。尽管所有基因的多态性水平都较低，但pif-2携带的多态性位点数量略多。总体而言，这项研究表明，经过二十年的反复田间应用后，pif基因一直保持稳定。