棉铃虫核型多角体病毒基因组变体的物理图谱与毒力

Physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants.

作者信息

Maruniak J E, Garcia-Maruniak A, Souza M L, Zanotto P M, Moscardi F

机构信息

Entomology and Nematology Department, University of Florida, Gainesville, Florida, USA.

出版信息

Arch Virol. 1999;144(10):1991-2006. doi: 10.1007/s007050050720.

DOI:10.1007/s007050050720

PMID:10550671

Abstract

Seventeen plaque purified isolates of two viral preparations of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), were analyzed in terms of the genomic changes after digestion of their DNAs with HindIII and PstI restriction enzymes. The 1979 AgMNPV wild type preparation (AgMNPV-'79) resulted in six different variants and the 1985 viral commercial preparation (AgMNPV-'85), in eleven. The genomic variation of all the isolates was mapped showing that those from 1985 presented more heterogeneity with changes mapped in additional sites in comparison to the AgMNPV-'79 variants. Their virulence was compared by infecting two Lepidopteran cell lines, Spodoptera frugiperda (IPLB-SF-21AE) and Anticarsia gemmatalis (UFL-AG-286). The results indicated that there was some difference in virulence within the AgMNPV-'85 variants. This commercial preparation had been applied in soybean fields in Brazil over several years to control the velvetbean caterpillar defoliation.

摘要

对斜纹夜蛾多核多角体病毒（AgMNPV）两种病毒制剂的17个噬菌斑纯化分离株，在用HindIII和PstI限制性内切酶消化其DNA后，分析了基因组变化。1979年的AgMNPV野生型制剂（AgMNPV-'79）产生了6种不同变体，1985年的病毒商业制剂（AgMNPV-'85）产生了11种。绘制了所有分离株的基因组变异图谱，结果表明，与AgMNPV-'79变体相比，1985年的分离株表现出更多的异质性，变异位点更多。通过感染两种鳞翅目细胞系，即草地贪夜蛾（IPLB-SF-21AE）和斜纹夜蛾（UFL-AG-286），比较了它们的毒力。结果表明，AgMNPV-'85变体的毒力存在一些差异。这种商业制剂已在巴西大豆田应用多年，用于控制豆蚀叶野螟的落叶。