Rackham Oliver, Filipovska Aleksandra
Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Level 5&6 MRF Building, Rear 50 Murray Street, 6000, Perth, WA, Australia.
Methods Mol Biol. 2014;1125:263-75. doi: 10.1007/978-1-62703-971-0_21.
RNA sequencing using next-generation technologies provides comprehensive coverage of transcriptomes at a much greater depth than conventional transcriptomic methods. The human mitochondrial genome is relatively small, and sequencing its transcriptome provides a valuable method to investigate changes in RNA metabolism in great detail. Here we describe two methods that use next-generation technologies to investigate mitochondrial RNAs. Directional RNA sequencing enables the analyses of RNA abundance from each strand of the mitochondrial DNA. Parallel analysis of RNA ends enables the analyses of processing of mitochondrial transcripts, their termini, and annotation of any new transcripts.
使用新一代技术进行RNA测序，能比传统转录组学方法更深入、全面地覆盖转录组。人类线粒体基因组相对较小，对其转录组进行测序为详细研究RNA代谢变化提供了一种有价值的方法。在此，我们描述两种利用新一代技术研究线粒体RNA的方法。定向RNA测序能够分析线粒体DNA每条链上的RNA丰度。RNA末端的平行分析能够分析线粒体转录本的加工过程、其末端以及任何新转录本的注释。
