The Genome Analysis Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK.
Trends Biotechnol. 2011 Nov;29(11):586-94. doi: 10.1016/j.tibtech.2011.06.004. Epub 2011 Jul 20.
The arrival of second-generation sequencing has revolutionized the study of bacteria within a short period. The sequence information generated from these platforms has helped in our understanding of bacterial development, adaptation and diversity and how bacteria cause disease. Furthermore, these technologies have quickly been adapted for high-throughput studies that were previously performed using DNA cloning or microarray-based applications. This has facilitated a more comprehensive study of bacterial transcriptomes through RNA sequencing (RNA-Seq) and the systematic determination of gene function by 'transposon monitoring'. In this review, we provide an outline of these powerful tools and the in silico analyses used in their application, and also highlight the biological questions being addressed in these approaches.
第二代测序技术的出现，在短时间内彻底改变了细菌研究。这些平台产生的序列信息帮助我们理解了细菌的发育、适应和多样性，以及细菌如何引起疾病。此外，这些技术已迅速适应于高通量研究，而这些高通量研究之前是使用 DNA 克隆或基于微阵列的应用程序来完成的。这通过 RNA 测序（RNA-Seq）促进了对细菌转录组的更全面研究，并通过“转座子监测”系统地确定了基因功能。在这篇综述中，我们提供了这些强大工具的概述以及在其应用中使用的计算机分析，并强调了这些方法所解决的生物学问题。
