Jeske Mandy, Temme Claudia, Wahle Elmar
Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120, Halle, Germany.
Methods Mol Biol. 2014;1125:297-311. doi: 10.1007/978-1-62703-971-0_24.
Deadenylation is the removal of poly(A) tails from mRNA. This chapter presents two methods to assay deadenylation in vitro. The first is a quick and quantitative assay for the degradation of radiolabeled poly(A) that can easily be adapted to be used for many different enzymes. The second method uses an extract from Drosophila embryos to catalyze the deadenylation of an RNA dependent on a specific sequence that also directs deadenylation in vivo.
去腺苷酸化是指从mRNA上去除多聚（A）尾巴。本章介绍了两种体外检测去腺苷酸化的方法。第一种是对放射性标记的多聚（A）降解进行快速定量检测，该方法可轻松适用于多种不同的酶。第二种方法使用果蝇胚胎提取物催化依赖特定序列的RNA的去腺苷酸化，该序列在体内也指导去腺苷酸化。
