Temme Claudia, Wahle Elmar
Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120, Halle, Germany.
Methods Mol Biol. 2014;1125:313-24. doi: 10.1007/978-1-62703-971-0_25.
Deadenylation is the removal of poly(A) tails from mRNA. Here, we present two methods for assaying deadenylation in vivo. The first is a method for measuring bulk poly(A) tail lengths. When combined with a block in transcription, the method can be used for measuring the rate of bulk poly(A) tail shortening. The second is an RT-PCR method to determine the poly(A) tail lengths of individual RNAs. Again in combination with a block of transcription, the method permits the rate of deadenylation of an individual RNA to be measured.
去腺苷酸化是指从mRNA上去除多聚（A）尾巴。在此，我们介绍两种在体内检测去腺苷酸化的方法。第一种是测量总体多聚（A）尾巴长度的方法。当与转录阻断相结合时，该方法可用于测量总体多聚（A）尾巴缩短的速率。第二种是用于确定单个RNA多聚（A）尾巴长度的RT-PCR方法。同样与转录阻断相结合，该方法可以测量单个RNA的去腺苷酸化速率。
