Takeuchi T, Schlossman S F, Morimoto C
Division of Tumor Immunobiology, Dana-Farber Cancer Institute, Boston, MA.
J Immunol. 1988 Nov 1;141(9):3010-5.
A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed alpha- and beta-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.
从用钥孔戚血蓝蛋白（KLH）进行超免疫的健康供体中产生了一个长期培养的依赖白细胞介素-2的KLH特异性CD8（T8）抑制性克隆（5B9）。5B9克隆群体在体外抑制了抗KLH抗体反应，但不抑制抗破伤风类毒素（TT）抗体反应或美洲商陆有丝分裂原（PWM）驱动的IgG合成。此外，即使存在游离的KLH，5B9细胞也不能抑制抗TT抗体反应。5B9克隆细胞抑制了与CD4 + 4B4 + 细胞一起培养的B细胞的抗KLH抗体反应，而不需要CD8 + 细胞的存在。这个KLH特异性CD8抑制性克隆是抑制性T细胞的效应器类型而非诱导器类型。克隆细胞在其细胞表面表达α和β-TCR蛋白（由WT-31抗体定义）。更重要的是，CD3:TCR复合物在该克隆诱导的抑制中具有功能重要性，因为从其细胞表面进行CD3抗原调节后，抑制效应功能被消除。
