Asano Y, Hodes R J
J Immunol. 1984 Dec;133(6):2864-7.
To determine the requirements underlying the antigen specificity observed in T cell-mediated immune response suppression, cloned major histocompatibility complex (MHC)-restricted T suppressor (Ts) cells specific for keyhole limpet hemocyanin (KLH) and cloned MHC-restricted T helper (Th) cells specific for fowl gamma-globulin (FGG) were employed to study the regulation of trinitrophenyl (TNP)-specific B cell responses. Neither antigen bridging between Ts cells and Th cells (FGG=KLH) nor bridging between Ts cells and B cells (TNP-KLH) was sufficient to allow suppression; a mixture of FGG=KLH and TNP-KLH was also insufficient for suppression. In contrast, suppression was induced by KLH-specific Ts cells only when suppressor determinants (KLH), helper determinants (FGG), and B cell determinants (TNP) were covalently linked on the same molecule (TMP-FGG)=(TNP-KLH) or TNP-(FGG=KLH)). These findings imply that a tripartite antigen-mediated interaction of Ts cells, Th cells, and responding B cells is necessary for the mediation of this antigen-specific suppression.
为了确定在T细胞介导的免疫反应抑制中所观察到的抗原特异性背后的必要条件，使用了对钥孔戚血蓝蛋白（KLH）特异的克隆化主要组织相容性复合体（MHC）限制性T抑制（Ts）细胞以及对鸡γ球蛋白（FGG）特异的克隆化MHC限制性T辅助（Th）细胞来研究三硝基苯（TNP）特异性B细胞反应的调节。无论是Ts细胞与Th细胞之间的抗原桥联（FGG=KLH）还是Ts细胞与B细胞之间的桥联（TNP-KLH）都不足以实现抑制；FGG=KLH与TNP-KLH的混合物也不足以实现抑制。相反，仅当抑制决定簇（KLH）、辅助决定簇（FGG）和B细胞决定簇（TNP）在同一分子上共价连接时（TMP-FGG）=（TNP-KLH）或TNP-（FGG=KLH）），由KLH特异性Ts细胞诱导出抑制。这些发现表明，Ts细胞、Th细胞和反应性B细胞之间的三方抗原介导的相互作用对于这种抗原特异性抑制的介导是必要的。
