Lüscher T F, Vanhoutte P M
Department of Physiology and Biophysics, Mayo Clinic, Rochester, Minnesota 55905.
J Cardiovasc Pharmacol. 1988;11 Suppl 1:S16-21.
Experiments were performed to examine the interaction(s) between alpha 1-adrenergic and S2-serotonergic receptors of vascular smooth muscle of the dog. Rings of isolated coronary and femoral arteries (without endothelium) were suspended for isometric tension recording in organ chambers filled with modified Krebs-Ringer bicarbonate solution. 5-Hydroxytryptamine (serotonin) augmented the responsiveness to norepinephrine in the coronary artery (log shift at ED50 of the concentration-response curve: 2.9-fold). In the femoral artery, the contractions to either norepinephrine or the partial alpha 1-adrenergic agonist St 587 were unchanged by serotonin. Ketanserin (10(-9) M) augmented the response to the full alpha 1-adrenergic agonist phenylephrine, but not to that of St 587; at 5 x 10(-9) M, it depressed the contractions to phenylephrine. Ketanserin did not affect the alpha 1-adrenergic blocking properties of prazosin. By contrast, prazosin, in a concentration selective for alpha 1-adrenergic receptors, potentiated the inhibitory effect of ketanserin on contractions evoked by serotonin. These experiments suggest different interactions between S2-serotonergic and alpha 1-adrenergic receptors in the canine coronary and femoral artery.
进行实验以研究犬血管平滑肌α1 - 肾上腺素能受体与5 - 羟色胺能受体之间的相互作用。将分离的冠状动脉和股动脉环（无内皮）悬挂在充满改良的克雷布斯 - 林格碳酸氢盐溶液的器官浴槽中，用于记录等长张力。5 - 羟色胺（血清素）增强了冠状动脉对去甲肾上腺素的反应性（浓度 - 反应曲线的ED50处的对数位移：2.9倍）。在股动脉中，血清素对去甲肾上腺素或部分α1 - 肾上腺素能激动剂St 587引起的收缩无影响。酮色林（10(-9) M）增强了对完全α1 - 肾上腺素能激动剂去氧肾上腺素的反应，但对St 587的反应无增强作用；在5×10(-9) M时，它抑制了对去氧肾上腺素的收缩反应。酮色林不影响哌唑嗪的α1 - 肾上腺素能阻断特性。相反，在对α1 - 肾上腺素能受体具有选择性的浓度下，哌唑嗪增强了酮色林对血清素引起的收缩的抑制作用。这些实验表明犬冠状动脉和股动脉中5 - 羟色胺能受体与α1 - 肾上腺素能受体之间存在不同的相互作用。
