Institute of Hematology and Transfusion Medicine, Warsaw, Poland.
Adv Clin Exp Med. 2014 Jan-Feb;23(1):127-33. doi: 10.17219/acem/37036.
Immunofixation (IFE) is a standard method for detecting monoclonal immunoglobulins and characterizing its isotype. Recently clonality can also be determined by using immunoglobulin (Ig) heavy chain/light chain immunoassays - HLC, HevyliteTM. HLC separately measures in pairs light chain types of each intact Ig class generating ratios of monoclonal Ig/uninvolved polyclonal Ig concentrations. Studies have shown that HLC and IFE are complementary methods. HLC assays quantify monoclonal proteins and identify monoclonality. It is possible to predict prognosis in multiple myeloma and to monitor response to treatment using HLC ratio. HLC ratio may serve as a parameter for myeloma induced immunoparesis and serve as a new marker for validating remission depth and relapse probabilities.
免疫固定电泳（IFE）是检测单克隆免疫球蛋白并对其同种型进行鉴定的标准方法。最近，使用免疫球蛋白（Ig）重链/轻链免疫分析- HLC、HevyliteTM 也可以确定克隆性。HLC 分别成对地测量每种完整 Ig 类别的轻链类型，产生单克隆 Ig/未涉及的多克隆 Ig 浓度的比值。研究表明，HLC 和 IFE 是互补的方法。HLC 检测定量单克隆蛋白并确定单克隆性。使用 HLC 比值可以预测多发性骨髓瘤的预后，并监测对治疗的反应。HLC 比值可作为骨髓瘤引起免疫功能低下的参数，并作为验证缓解深度和复发概率的新标志物。
