Cao Gang, Cai Hao, Jiang Jianping, Yao Linjiang, Tu Sicong, Wang Lin, Ma Xiaoqing, Cai Baochang
Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R. China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
J Sep Sci. 2014 May;37(9-10):1194-8. doi: 10.1002/jssc.201301376. Epub 2014 Apr 10.
In the present study, comprehensive 2D GC-TOF-MS combined with multivariate data analysis was applied to analyze the differences of the volatile components in crude and processed Atractylodis Macrocephalae Rhizoma (AMR) samples. As a result, 26 compounds that were found in crude AMR samples disappeared in processed AMR samples, and 19 compounds were newly generated and identified in AMR after processing with wheat bran. Meanwhile, principal component analysis demonstrated that there were significant chemical differences between crude and processed AMR samples, and processing procedure caused obvious quantitative and qualitative changes of volatile components in AMR. The established method could be used to explain the chemical differentiation between crude and processed AMR, and to further understand the processing mechanism of herbal medicines.
在本研究中，采用全二维气相色谱-飞行时间质谱联用技术结合多变量数据分析，对生白术和麸炒白术样品中的挥发性成分差异进行分析。结果显示，生白术样品中发现的26种化合物在麸炒白术样品中消失，且在用麦麸炮制后的白术中，新生成并鉴定出19种化合物。同时，主成分分析表明，生白术和麸炒白术样品之间存在显著的化学差异，炮制过程导致白术挥发性成分发生明显的定量和定性变化。所建立的方法可用于解释生白术和麸炒白术之间的化学差异，并进一步了解中药材的炮制机制。
