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[用辣根过氧化物酶研究幼鼠离体脊髓中运动神经元相互作用的形态学基础]

[Morphological bases of motor neuron interaction in the isolated spinal cord of rat pups studied with horseradish peroxidase].

作者信息

Chmykhova N M, Karamian O A, Kozhanov V M

出版信息

Neirofiziologiia. 1988;20(3):340-50.

PMID:2459623
Abstract

The structure of connections of the lumbar motoneurons was investigated in the isolated spinal cord of young rats by the HRP methods. HRP was applied to the ventral roots and injected intracellularly into the motoneurons. The light microscopic study has shown that dendrodendritic, dendrosomatic and somatosomatic contacts between motoneurons might be established. Recurrent collaterals of motor axons were revealed. They were found to form contacts on the motoneuron dendrites and perikarya. The course and terminal arborizations of the ventral root afferent fibre were also described. The obtained morphological data related to electrophysiological analysis of motoneuronal postsynaptic potentials evoked by ventral root stimulation are discussed.

摘要

采用辣根过氧化物酶(HRP)法,在幼鼠离体脊髓中研究腰段运动神经元的连接结构。将HRP应用于腹根,并通过细胞内注射到运动神经元中。光学显微镜研究表明,运动神经元之间可能建立树突 - 树突、树突 - 胞体和胞体 - 胞体联系。发现了运动轴突的回返侧支,它们在运动神经元的树突和胞体上形成联系。还描述了腹根传入纤维的走行和终末分支。讨论了所获得的形态学数据与腹根刺激诱发的运动神经元突触后电位的电生理分析的关系。

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