Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA.
Environ Microbiol Rep. 2014 Feb;6(1):57-69. doi: 10.1111/1758-2229.12108. Epub 2013 Oct 21.
Associations among insects and microbes can lead to beneficial or parasitic interactions. Using 454 sequencing of 16S RNA genes, we compared microbiome diversity and abundance among field-collected (F) and laboratory-reared (L) populations of the invasive soybean aphid (Aphis glycines), a pest of soybean. Additionally, we screened A. glycines populations from native (Japan, South Korea and China) and invasive regions (North America) to broadly determine the microbiome diversity. Our results suggested that Arsenophonus (relative abundance of 54.6%), Buchnera (38.7%) and Wolbachia (3.7%) were the major bacteria associated with A. glycines. Arsenophonus was the most abundant in F populations but was significantly reduced in L populations; additional bacteria species also had lower relative abundances in L populations. Native and invasive populations were largely similar in bacteria communities and revealed substantial superinfection of Arsenophonus and Wolbachia. The lone exception was a lack of Arsenophonus in A. glycines from Japan. Divergent selection pressures among natural and laboratory populations were inferred as factors driving the differential bacterial communities observed. Our results will allow for improved comparative aphid-symbiont research and broaden our understanding of the interactions among insects, endosymbionts and their environments.
昆虫和微生物之间的相互关系可能导致有益或寄生的相互作用。我们使用 16S RNA 基因的 454 测序技术，比较了野外采集（F）和实验室饲养（L）的入侵性大豆蚜虫（Aphis glycines）种群的微生物组多样性和丰度，大豆的害虫。此外，我们从原产（日本、韩国和中国）和入侵地区（北美）筛选了 A. glycines 种群，以广泛确定微生物组多样性。我们的结果表明，Arsenophonus（相对丰度为 54.6%）、Buchnera（38.7%）和Wolbachia（3.7%）是与 A. glycines 相关的主要细菌。Arsenophonus 在 F 种群中最为丰富，但在 L 种群中显著减少；其他细菌种类在 L 种群中的相对丰度也较低。在细菌群落中，本地和入侵种群基本相似，并且显示出 Arsenophonus 和 Wolbachia 的大量超级感染。唯一的例外是日本的 A. glycines 中缺乏 Arsenophonus。自然和实验室种群之间的不同选择压力被推断为导致观察到的细菌群落差异的因素。我们的研究结果将有助于改进比较蚜虫-共生体研究，并拓宽我们对昆虫、内共生体及其环境之间相互作用的理解。
