Li T, Xiao J H, Wu Y Q, Huang D W
Natural Enemy Research Group, Institute of Plant Protection, Henan Academy of Agricultural Science, Zhengzhou 450002, China.
Environ Entomol. 2014 Jun;43(3):605-11. doi: 10.1603/EN13229.
Aphids are a group of insects frequently associated with bacterial symbionts. Although Chinese aphids harbor a high level of species diversity, the associations between Chinese aphids and bacterial symbionts are less known. In this study, we uncovered the diversity of bacterial symbionts in a Chinese widespread aphid, Sitobion miscanthi (Takahashi). In this study, we detected the aphid obligate symbiont Buchnera aphidicola, and two secondary symbionts, Hamiltonella defensa and Regiella insecticola, with the diagnostic polymerase chain reaction method in S. miscanthi samples. In addition, symbiotic species of Acinetobacter, Aeromonas, Enterobacter, Pantoea, and Pseudomonas, and the family Enterobacteriaceae were also found. Geographically, sporadic occurrences were detected for H. defensa and R. insecticola. Moreover, the infection rates of them vary widely among the infected populations: H. defensa (5.26-95.2%) and R. insecticola (5.26-46.7%). Phylogenetic analyses indicated that the strain of B. aphidicola mirrored the history and divergence of S. miscanthi; however, the H. defensa and R. insecticola strains were probably experienced horizontal transmission among S. miscanthi and its distantly related species.
蚜虫是一类常与细菌共生体相关联的昆虫。尽管中国的蚜虫具有高度的物种多样性，但中国蚜虫与细菌共生体之间的关联却鲜为人知。在本研究中，我们揭示了中国广泛分布的一种蚜虫——荻草谷网蚜（Sitobion miscanthi (Takahashi)）体内细菌共生体的多样性。在本研究中，我们采用诊断性聚合酶链反应方法，在荻草谷网蚜样本中检测到了蚜虫专性共生体蚜虫内共生菌（Buchnera aphidicola），以及两种次生共生体——防御汉氏菌（Hamiltonella defensa）和昆虫雷氏菌（Regiella insecticola）。此外，还发现了不动杆菌属、气单胞菌属、肠杆菌属、泛菌属、假单胞菌属的共生菌种以及肠杆菌科。在地理分布上，防御汉氏菌和昆虫雷氏菌呈零星分布。而且，它们在受感染种群中的感染率差异很大：防御汉氏菌为5.26% - 95.2%，昆虫雷氏菌为5.26% - 46.7%。系统发育分析表明，蚜虫内共生菌的菌株反映了荻草谷网蚜的历史和分化；然而，防御汉氏菌和昆虫雷氏菌的菌株可能在荻草谷网蚜及其远缘物种之间经历了水平传播。
