Department of Physiology, Medical School, National & Kapodistrian University of Athens, 75 Micras Asias, Goudi, Athens, 115 27, Greece.
Anticancer Res. 2014 Mar;34(3):1401-7.
Numerous studies have shown that the Kiss-1 gene countervails the metastatic aptitude of several cancer cell lines and solid-tumor neoplasias. However, there still remains ambiguity regarding its role in breast cancer and literature has arisen asserting that Kiss-1 expression may be linked to an aggressive phenotype and malignant progression. Herein, we investigated the protein expression of Kiss-1 and its receptor GPR54 in breast cancer tissues compared to non-cancerous mammary tissues.
Paraffin-fixed cancer tissues from 43 women with resected breast adenocarcinomas and 11 specimens derived from women suffering from fibrocystic disease, serving as controls, were immunostained with Kiss-1 and GPR54 antibodies.
Kiss-1 and GPR54 protein expression levels were significantly higher in breast cancer compared to fibrocystic tissues (p<0.05). No significant correlation was established between Kiss-1 or GRP54 expression and tumor grade, tumor size, lymph node positivity, histological type or ER status. Kiss-1 expression significantly and positively correlated with GPR54 expression in both breast cancer and fibrocystic disease specimens.
Kiss-1/GPR54 expression was found to be significantly higher in breast cancer compared to non-malignant mammary tissues.
许多研究表明 Kiss-1 基因可抑制多种癌细胞系和实体瘤的转移能力。然而，其在乳腺癌中的作用仍存在争议，有文献声称 Kiss-1 表达可能与侵袭性表型和恶性进展有关。在此，我们研究了 Kiss-1 及其受体 GPR54 在乳腺癌组织与非癌性乳腺组织中的蛋白表达情况。
使用 Kiss-1 和 GPR54 抗体对 43 例接受乳腺癌切除术的女性的癌组织和 11 例纤维囊性疾病女性的组织进行免疫染色。
与纤维囊性组织相比，乳腺癌组织中 Kiss-1 和 GPR54 蛋白表达水平显著升高（p<0.05）。Kiss-1 或 GRP54 表达与肿瘤分级、肿瘤大小、淋巴结阳性、组织学类型或 ER 状态之间无显著相关性。在乳腺癌和纤维囊性疾病标本中，Kiss-1 表达与 GPR54 表达呈显著正相关。
与非恶性乳腺组织相比，乳腺癌中 Kiss-1/GPR54 的表达显著升高。
