Markiewicz H, Krumrych W, Gehrke M
Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, Al. Powstańców Wlkp. 10, 85-090 Bydgoszcz, Poland.
Department of Animal Reproduction and Animal Health Protection, University of Technology and Life Sciences, 85-084 Bydgoszcz, Poland.
Pol J Vet Sci. 2013;16(4):671-7. doi: 10.2478/pjvs-2013-0095.
The aim of this field study was to assess the impact of a single i.m. injection of lysozyme dimer and flunixin meglumine in combination with intramammary and systemic antibiotic on chemiluminescence of PMN (polymorphonuclear leucocytes) and subpopulations of lymphocyte T in blood of cows with E. coli mastitis. Examinations were performed on 30 dairy cows affected with naturally occurring acute form of E. coli mastitis. Cows were randomly divided into three groups according to the method of treatment. The first group was treated with approved intramammary antibiotic product, the same antibiotic in i.m. injection and one injection of flunixin meglumine on the first day of therapy. Next group was treated with the same antibiotic and additionally one injection of lysozyme dimer on the first day of therapy. The third one was treated only with an antibiotic and served as a control group. Blood samples were taken before treatment and on days 3 and 7. In samples haematology indices were determined, spontaneous and opsonised zymosan stimulated CL and PMA measurements were performed and the subpopulations of T lymphocyte (CD2(+), CD4(+), CD8(+)) were assayed in whole blood. There was no effect of the applied supportive treatment on the value of morphological blood indices. A significant influence of the time of sample collection on the level of CL and dynamics of lymphocytes T subpopulation was demonstrated. A single injection of flunixin meglumine or lysozyme dimer on the day of the beginning of treatment of E. coli mastitis, does not affect the level of neutrophil chemiluminescence and the percentage of T lymphocytes in the blood of mastitic cows in the analysed period of time.
本田间研究的目的是评估单次肌内注射溶菌酶二聚体和氟尼辛葡甲胺联合乳房内和全身用抗生素对患有大肠杆菌性乳腺炎奶牛血液中PMN（多形核白细胞）化学发光和T淋巴细胞亚群的影响。对30头患有自然发生的急性大肠杆菌性乳腺炎的奶牛进行了检查。根据治疗方法将奶牛随机分为三组。第一组用批准的乳房内抗生素产品治疗，在治疗第一天肌内注射相同抗生素并注射一次氟尼辛葡甲胺。下一组用相同抗生素治疗，并且在治疗第一天额外注射一次溶菌酶二聚体。第三组仅用抗生素治疗并作为对照组。在治疗前以及第3天和第7天采集血样。测定样本中的血液学指标，进行自发和调理酵母聚糖刺激的化学发光以及佛波酯测量，并在全血中检测T淋巴细胞亚群（CD2(+)、CD4(+)、CD8(+)）。所应用的支持性治疗对血液形态学指标的值没有影响。证明了样本采集时间对化学发光水平和T淋巴细胞亚群动态有显著影响。在大肠杆菌性乳腺炎治疗开始当天单次注射氟尼辛葡甲胺或溶菌酶二聚体，在分析时间段内不影响患乳腺炎奶牛血液中中性粒细胞化学发光水平和T淋巴细胞百分比。
