Szulowski K, Iwaniak W, Weiner M, Zlotnicka J
Department of Microbiology of the National Veterinary Research Institute Al. Partyzantow 57, 24-100 Pulawy, Poland.
Pol J Vet Sci. 2013;16(4):757-8. doi: 10.2478/pjvs-2013-0109.
A total of 42 Brucella strains were isolated from animals in Poland in years 2003-2012. Most of them (N=37) originated from wild animals, 3 from cattle, 1 from pig and 1 from sheep. The strains were characterised using both bacteriological and molecular (Bruce-ladder and MLVA) methods. The examinations revealed that all strains from wild boars, hares, cattle and pigs (N=41) had the same phenotypic characteristics and were classified as B. suis biovar 2. The remaining strain, isolated from sheep, was classified as B. ovis. The molecular examination showed that all B. suis biovar 2 strains, except one, had the same molecular profile as reference strain B. suis bv2 Thomsen. Different from the others strain originated from boars imported to Poland and its VNTR profile was typical forIberian strains.
2003年至2012年期间，波兰共从动物身上分离出42株布鲁氏菌菌株。其中大多数（N = 37）源自野生动物，3株来自牛，1株来自猪，1株来自羊。使用细菌学和分子（布鲁斯梯和MLVA）方法对这些菌株进行了鉴定。检查发现，所有来自野猪、野兔、牛和猪的菌株（N = 41）具有相同的表型特征，被归类为猪布鲁氏菌生物变种2。其余从羊身上分离出的菌株被归类为绵羊布鲁氏菌。分子检测表明，除一株外，所有猪布鲁氏菌生物变种2菌株的分子图谱与参考菌株猪布鲁氏菌bv2汤姆森相同。与其他菌株不同的是，该菌株源自进口到波兰的野猪，其VNTR图谱是伊比利亚菌株的典型图谱。
