意大利中北部育肥猪群中使用标准程序、斑点印迹法和蛋白质印迹法进行猪布鲁氏菌病血清学调查
A Serological Survey on Swine Brucellosis Using Standard Procedures, Dot Blot, and Western Blot in Finisher Pigs in Central-North Italy.
作者信息
Bertelloni Fabrizio, Forzan Mario, Turchi Barbara, Sagona Simona, Mazzei Maurizio, Felicioli Antonio, Fratini Filippo, Cerri Domenico
机构信息
Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
出版信息
Vet Sci. 2018 Oct 3;5(4):86. doi: 10.3390/vetsci5040086.
In recent years, has been sporadically reported in Italy in domestic and wild swine. Since standard serological tests can determine false positive results, the development of alternative tests with improved sensitivity and specificity is rather essential. We analyzed 1212 sera collected at slaughterhouse from healthy pigs belonging to 62 farms of North-Central Italy. Sera were tested by Rose Bengal Test, Complement Fixation Test, and subsequently by a Dot Blot (DB) and Western Blot assays (WB). Only one serum resulted positive to all tests, indicating that swine brucellosis has a very limited spread. DB and WB could represent a support to the available serological tests; however, further studies to validate these tests are needed. In the presence of reemerging diseases, a prompt and continuous monitoring design is necessary to acquire epidemiological information for the subsequent application of specific health emergency plans.
近年来，意大利国内和野猪中偶尔有相关报道。由于标准血清学检测可能会得出假阳性结果，因此开发具有更高灵敏度和特异性的替代检测方法至关重要。我们分析了从意大利中北部62个农场的健康猪在屠宰场采集的1212份血清。血清通过玫瑰红试验、补体结合试验进行检测，随后通过斑点印迹（DB）和蛋白质印迹分析（WB）进行检测。只有一份血清对所有检测均呈阳性，这表明猪布鲁氏菌病的传播非常有限。DB和WB可为现有的血清学检测提供支持；然而，需要进一步研究来验证这些检测方法。在出现重新出现的疾病时，有必要进行及时且持续的监测设计，以获取流行病学信息，以便随后应用特定的卫生应急计划。
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