Zhang J, Huether G, Neuhoff V
Max-Planck-Institute for Experimental Medicine, Department of Neurochemistry, Gottingen, F.R.G.
Int J Dev Neurosci. 1987;5(2):151-65. doi: 10.1016/0736-5748(87)90061-x.
The developmental changes in the regional protein patterns of the embryonic brain of chick were analysed by micro-two-dimensional polyacrylamide gel electrophoresis stained by a highly sensitive procedure with Coomassie Brilliant Blue G-250 that completely avoids background staining. More than 400 protein spots were resolved in a computerized plot. Many proteins of the various embryonic brain regions varied with age. At least six of these proteins changed remarkably during development. One of them (Mr 14K) was prominent at 6 days and disappeared after 12 days of incubation. One acidic protein (Mr 30K) appeared at 12 days together with two acidic protein spots near tubulin and increased with development. A group of more basic proteins (Mr 94K) was prominent at 6 day incubation and decreased during development. One more basic protein (Mr 20K) was first observed at 18 days of incubation in the metencephalon. It was identified as myelin basic protein, and strikingly increased in all brain regions until hatching. A group of basic proteins with high molecular weight (Mr 94K) appeared at day 12 of incubation, and increased remarkably before hatching. They were identified to be collagen associated with brain capillary vessels. Stimulation by intermittent light in ovo from day 10 to day 16 caused a retarded appearance of these collagen proteins, and an increased formation of tubulin in the optic lobe of the developing chick embryo.
采用考马斯亮蓝G - 250高灵敏度染色程序进行微二维聚丙烯酰胺凝胶电泳，该程序完全避免背景染色，以此分析鸡胚脑区域蛋白质模式的发育变化。在计算机化图谱中分辨出400多个蛋白质斑点。不同胚胎脑区域的许多蛋白质随年龄变化。其中至少六种蛋白质在发育过程中变化显著。其中一种（分子量14K）在孵化6天时显著，孵化12天后消失。一种酸性蛋白质（分子量30K）在12天时出现，同时在微管蛋白附近出现两个酸性蛋白质斑点，并随发育增加。一组碱性更强的蛋白质（分子量94K）在孵化6天时显著，发育过程中减少。另一种碱性更强的蛋白质（分子量20K）首次在孵化18天时于后脑被观察到。它被鉴定为髓鞘碱性蛋白，在所有脑区域直至孵化时显著增加。一组高分子量碱性蛋白质（分子量94K）在孵化12天时出现，孵化前显著增加。它们被鉴定为与脑毛细血管相关的胶原蛋白。在卵内从第10天到第16天进行间歇性光照刺激，导致这些胶原蛋白延迟出现，并使发育中的鸡胚视叶中微管蛋白的形成增加。
