Shirao T, Obata K
J Neurochem. 1985 Apr;44(4):1210-6. doi: 10.1111/j.1471-4159.1985.tb08745.x.
The developmental changes in protein composition of the chick optic tectum were analyzed by two-dimensional gel electrophoresis. Staining with Coomassie Brilliant Blue R revealed 54 major proteins, eight of which remarkably changed their abundance during development: Four of these proteins (S8, S14, S30, and S54) increased and two of them (S7 and S37) decreased in the course of the brain development. The other two proteins (S5 and S6) appeared at specific embryonic stages and were not detected in the adult. The abundance of S5 protein was highest at day 7, and that of S6 protein at days 9-18. The two proteins were present in other regions of the embryonic brain but were not detected in the embryonic liver. The proteins were purified from the soluble fraction of embryonic chick brains by pH 5.5 precipitation, DEAE-Sepharose column chromatography, ammonium sulfate precipitation, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weights of S5 and S6 proteins were 95,000 and 100,000, respectively, and their isoelectric points were about 4.5. They were compared by peptide mapping using V8 protease and found to share 11 common peptides out of 17 distinct ones. This indicates a strong degree of structural homology between these two proteins.
通过二维凝胶电泳分析了鸡视顶盖蛋白质组成的发育变化。用考马斯亮蓝R染色显示有54种主要蛋白质，其中8种在发育过程中其丰度发生了显著变化：在脑发育过程中，这些蛋白质中有4种（S8、S14、S30和S54）增加，2种（S7和S37）减少。另外两种蛋白质（S5和S6）出现在特定的胚胎阶段，在成体中未检测到。S5蛋白的丰度在第7天最高，S6蛋白的丰度在第9至18天最高。这两种蛋白质存在于胚胎脑的其他区域，但在胚胎肝脏中未检测到。通过pH 5.5沉淀、DEAE-琼脂糖柱色谱、硫酸铵沉淀和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳从胚胎鸡脑的可溶性部分中纯化这些蛋白质。S5和S6蛋白的分子量分别为95,000和100,000，其等电点约为4.5。使用V8蛋白酶通过肽图谱分析对它们进行比较，发现在17个不同的肽中有11个共同肽。这表明这两种蛋白质之间存在高度的结构同源性。
