Tsai Cheng-Fang, Yeh Wei-Lan, Chen Jia-Hong, Lin Chingju, Huang Shiang-Suo, Lu Dah-Yuu
Department of Biotechnology, Asia University, Taichung 41354, Taiwan.
Department of Cell and Tissue Engineering, Changhua Christian Hospital, Changhua 50006, Taiwan.
Int J Mol Sci. 2014 Mar 4;15(3):3889-903. doi: 10.3390/ijms15033889.
Glioblastoma multiforme (GBM) is the most common type of primary and malignant tumor occurring in the adult central nervous system. GBM often invades surrounding regions of the brain during its early stages, making successful treatment difficult. Osthole, an active constituent isolated from the dried C. monnieri fruit, has been shown to suppress tumor migration and invasion. However, the effects of osthole in human GBM are largely unknown. Focal adhesion kinase (FAK) is important for the metastasis of cancer cells. Results from this study show that osthole can not only induce cell death but also inhibit phosphorylation of FAK in human GBM cells. Results from this study show that incubating GBM cells with osthole reduces matrix metalloproteinase (MMP)-13 expression and cell motility, as assessed by cell transwell and wound healing assays. This study also provides evidence supporting the potential of osthole in reducing FAK activation, MMP-13 expression, and cell motility in human GBM cells.
多形性胶质母细胞瘤（GBM）是成人中枢神经系统中最常见的原发性恶性肿瘤类型。GBM在早期阶段常侵袭脑周围区域，导致成功治疗困难。蛇床子素是从干燥的蛇床果实中分离出的一种活性成分，已被证明可抑制肿瘤迁移和侵袭。然而，蛇床子素对人GBM的影响在很大程度上尚不清楚。粘着斑激酶（FAK）对癌细胞转移很重要。本研究结果表明，蛇床子素不仅能诱导细胞死亡，还能抑制人GBM细胞中FAK的磷酸化。本研究结果表明，用蛇床子素处理GBM细胞可降低基质金属蛋白酶（MMP）-13的表达和细胞运动性，这是通过细胞穿膜和伤口愈合试验评估得出的。本研究还提供了证据，支持蛇床子素在降低人GBM细胞中FAK激活、MMP-13表达和细胞运动性方面的潜力。
