Lin Chin-Chung, Chen Jin-Tang, Yang Jai-Sing, Lu Hsu-Feng, Hsu Shu-Chun, Tan Tzu-Wei, Lin Yuh-Tzy, Ma Yi-Shih, Ip Siu-Wan, Wu Jia-Jiuan, Li Yu-Chinh, Chung Jing-Gung
Fong-Yuan Hospital, Department of Health, Executive Yuan, Fong Yuan 402, Taiwan, ROC.
Oncol Rep. 2009 Nov;22(5):1033-7. doi: 10.3892/or_00000532.
In this study, we investigated the effect of danthron on the cell migration and invasion of human brain glioblastoma multiforme GBM 8401 cells in vitro. The changes of migration and invasion of GBM 8401 cells after treatment with danthron were detected by cell migration assay and cell invasion assay. The levels of mRNA gene expression associated with cell migration and invasion were detected by real-time PCR. Results indicated that human brain glioblastoma multiforme GBM 8401 cells treated with danthron in vitro migrated and invaded less than cells treated with phosphate-buffered saline (PBS) (control). Western blotting showed that danthron inhibited the protein levels of FAK, MMP-7, MMP-9 and uPA in GBM 8401 cells. Real-time PCR assay also showed that danthron inhibited the mRNA expression of matrix metalloproteinase-9 (MMP-9), FAK and ROCK-1 of GBM 8401 cells. These results showed that danthron inhibited invasion and migration of GBM 8401 cells by downregulating mRNA expression associated with these processes, resulting in reduced metastasis. Thus, danthron may be considered a therapeutic agent that can inhibit primary tumor growth and prevent metastasis.
在本研究中，我们在体外研究了大黄素对人多形性胶质母细胞瘤GBM 8401细胞迁移和侵袭的影响。通过细胞迁移试验和细胞侵袭试验检测大黄素处理后GBM 8401细胞迁移和侵袭的变化。通过实时PCR检测与细胞迁移和侵袭相关的mRNA基因表达水平。结果表明，体外经大黄素处理的人多形性胶质母细胞瘤GBM 8401细胞的迁移和侵袭能力低于经磷酸盐缓冲盐水（PBS）处理的细胞（对照）。蛋白质印迹法显示，大黄素抑制GBM 8401细胞中FAK、MMP-7、MMP-9和uPA的蛋白水平。实时PCR分析还显示，大黄素抑制GBM 8401细胞中基质金属蛋白酶-9（MMP-9）、FAK和ROCK-1的mRNA表达。这些结果表明，大黄素通过下调与这些过程相关的mRNA表达来抑制GBM 8401细胞的侵袭和迁移，从而减少转移。因此，大黄素可被视为一种能够抑制原发性肿瘤生长并预防转移的治疗药物。
