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C57BL/6N白化/刺鼠突变小鼠作为胚胎供体用于C57BL/6胚胎干细胞的高效种系传递。

C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells.

作者信息

Zevnik Branko, Uyttersprot Nathalie C, Perez Ana V, Bothe Gerald W M, Kern Heidrun, Kauselmann Gunther

机构信息

Cologne Excellence Cluster on Cellular Stress Responses in Aging-associated Diseases (CECAD), University of Cologne, Cologne, Germany.

TaconicArtemis GmbH, Cologne, Germany.

出版信息

PLoS One. 2014 Mar 5;9(3):e90570. doi: 10.1371/journal.pone.0090570. eCollection 2014.

DOI:10.1371/journal.pone.0090570
PMID:24599260
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3944090/
Abstract

We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells.

摘要

我们培育出了携带酪氨酸酶功能缺失突变且非刺鼠位点回复为刺鼠的C57BL/6NTac小鼠。该品系具有高超排卵反应，能通过视觉检测C57BL/6胚胎干细胞对嵌合体毛色的贡献，并且提供了一种简化的育种方式，可一步产生纯合C57BL/6背景的黑色G1代后代，为基因操作的C57BL/6胚胎干细胞提供了理想的宿主。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/72033900ca9f/pone.0090570.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/e3f626f6dfe9/pone.0090570.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/cc4c89afd840/pone.0090570.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/7ecc7061e123/pone.0090570.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/72033900ca9f/pone.0090570.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/e3f626f6dfe9/pone.0090570.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/cc4c89afd840/pone.0090570.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/7ecc7061e123/pone.0090570.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b70/3944090/72033900ca9f/pone.0090570.g004.jpg

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2
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Transgenic Res. 2012 Dec;21(6):1149-58. doi: 10.1007/s11248-012-9605-3. Epub 2012 Mar 16.
3
A conditional knockout resource for the genome-wide study of mouse gene function.
B6(Cg)-Tyr /J 和 C57BL/6NTac 胚胎作为基因敲除小鼠模型生成宿主的效率比较。
Transgenic Res. 2021 Jun;30(3):275-281. doi: 10.1007/s11248-021-00248-9. Epub 2021 Apr 12.
4
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5
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Nature. 2011 Jun 15;474(7351):337-42. doi: 10.1038/nature10163.
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