Madan E, Kemp J, Westblom T U, Subik M, Sexton S, Cook J
Marshall University School of Medicine, Department of Pathology, Huntington, West Virginia 25702.
Am J Clin Pathol. 1988 Oct;90(4):450-3. doi: 10.1093/ajcp/90.4.450.
Campylobacter pylori has been implicated in the pathogenesis of peptide ulcer disease. The rapid identification of this organism may depend upon histologic diagnosis, because culture methods are complex and require a minimum of seven days in order to identify a negative specimen. The purpose of this study was to determine which stain used to identify this organism was the most cost-effective and easiest to perform and interpret on a routine basis. Sixty-one consecutive gastric antral biopsies were stained with hematoxylin and eosin, Giemsa, Brown-Brenn, and Warthin-Starry, with 23 of the cases stained by Brown-Hopps. Of the stains tested, the Wright-Giemsa was the easiest to perform. The organisms on the Wright-Giemsa showed a smooth, uniform purple color, whereas the Warthin-Starry gave the organism a granular appearance that at times could be confused for silver precipitate. Both the Wright-Giemsa and Brown-Hopps stain had the highest degree of identification of the organism (defined by percent positivity). The routine use of the Wright-Giemsa stain for identification of C. pylori in antral biopsies is recommended.
幽门螺杆菌与消化性溃疡病的发病机制有关。该菌的快速鉴定可能依赖于组织学诊断，因为培养方法复杂，且为鉴定阴性标本至少需要7天时间。本研究的目的是确定用于鉴定该菌的哪种染色方法在常规操作中最具成本效益且最易于实施和解读。对61例连续的胃窦活检标本进行苏木精-伊红染色、吉姆萨染色、布朗-布伦染色和沃辛-斯塔瑞染色，其中23例采用布朗-霍普斯染色。在所测试的染色方法中，瑞氏-吉姆萨染色最易于实施。瑞氏-吉姆萨染色显示的菌呈现光滑、均匀的紫色，而沃辛-斯塔瑞染色使菌呈现颗粒状外观，有时可能会被误认为是银沉淀。瑞氏-吉姆萨染色和布朗-霍普斯染色对该菌的鉴定程度最高（以阳性百分比定义）。建议常规使用瑞氏-吉姆萨染色来鉴定胃窦活检标本中的幽门螺杆菌。
