Stanton Morgan M, Rankenberg Johanna M, Park Byung-Wook, McGimpsey W Grant, Malcuit Christopher, Lambert Christopher R
Worcester Polytechnic Institute, Department of Chemistry and Biochemistry, 100 Institute Road, Worcester, MA 01609, USA.
Macromol Biosci. 2014 Jul;14(7):953-64. doi: 10.1002/mabi.201300504. Epub 2014 Mar 6.
Designing complex tissue culture systems requires cell alignment and directed extracellular matrix (ECM) and gene expression. Here, a micro-rough, polydimethylsiloxane (PDMS) surface, that also integrates a micro-pattern of 50 µm wide lines of fibronectin (FN) separated by 60 µm wide lines of bovine serum albumin (BSA), is developed. Human fibroblasts cultured on the rough, patterned substrate have aligned growth and a significant change in morphology when compared to cells on a flat, patterned surface. The rough PDMS topography significantly decreases cell area and induces the upregulation of several ECM related genes by two-fold when compared to cells cultured on flat PDMS. This study describes a simple surface engineering procedure for creating surface architecture for scaffolds to design and control the cell-surface interface.
设计复杂的组织培养系统需要细胞排列以及定向的细胞外基质（ECM）和基因表达。在此，开发了一种微粗糙的聚二甲基硅氧烷（PDMS）表面，该表面还整合了由60μm宽的牛血清白蛋白（BSA）线分隔的50μm宽的纤连蛋白（FN）线的微图案。与在平坦的图案化表面上的细胞相比，在粗糙的图案化基质上培养的人成纤维细胞具有排列的生长和显著的形态变化。与在平坦的PDMS上培养的细胞相比，粗糙的PDMS拓扑结构显著减小了细胞面积，并使几种ECM相关基因的上调倍数达到两倍。本研究描述了一种简单的表面工程程序，用于为支架创建表面结构，以设计和控制细胞-表面界面。
