Stanton Morgan M, Parrillo Allegra, Thomas Gawain M, McGimpsey W Grant, Wen Qi, Bellin Robert M, Lambert Christopher R
Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, Massachusetts.
J Biomed Mater Res B Appl Biomater. 2015 May;103(4):861-9. doi: 10.1002/jbm.b.33244. Epub 2014 Aug 20.
The immediate physical and chemical surroundings of cells provide important biochemical cues for their behavior. Designing and tailoring biomaterials for controlled cell signaling and extracellular matrix (ECM) can be difficult due to the complexity of the cell-surface relationship. To address this issue, our research has led to the development of a polydimethylsiloxane (PDMS) scaffold with defined microtopography and chemistry for surface driven ECM assembly. When human fibroblasts were cultured on this microtextured PDMS with 2-6 µm wide vertical features, significant changes in morphology, adhesion, actin cytoskeleton, and fibronectin generation were noted when compared with cells cultured on unmodified PDMS. Investigation of cellular response and behavior was performed with atomic force microscopy in conjunction with fluorescent labeling of focal adhesion cites and fibronectin in the ECM. Changes in the surface topography induced lower adhesion, an altered actin cytoskeleton, and compacted units of fibronectin similar to that observed in vivo. Overall, these findings provide critical information of cell-surface interactions with a microtextured, polymer substrate that can be used in the field of tissue engineering for controlling cellular ECM interactions.
细胞所处的直接物理和化学环境为其行为提供了重要的生化线索。由于细胞与表面关系的复杂性，设计和定制用于控制细胞信号传导和细胞外基质（ECM）的生物材料可能具有挑战性。为了解决这个问题，我们的研究开发了一种具有特定微观形貌和化学性质的聚二甲基硅氧烷（PDMS）支架，用于表面驱动的ECM组装。当人成纤维细胞在这种具有2-6微米宽垂直特征的微纹理PDMS上培养时，与在未修饰的PDMS上培养的细胞相比，在形态、粘附、肌动蛋白细胞骨架和纤连蛋白生成方面观察到显著变化。利用原子力显微镜结合ECM中粘着斑位点和纤连蛋白的荧光标记对细胞反应和行为进行了研究。表面形貌的变化导致较低的粘附力、肌动蛋白细胞骨架改变以及类似于体内观察到的纤连蛋白紧密单元。总体而言，这些发现提供了细胞与微纹理聚合物底物表面相互作用的关键信息，可用于组织工程领域以控制细胞与ECM的相互作用。
