Campbell J R, Paleologo F P, Franke E D, Ratiwayanto S, Hadiputranto H, Kurniawan L, Wistar R, Hoffman S L, Annis B A, Wasserman G
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.
Am J Trop Med Hyg. 1988 Sep;39(3):232-5. doi: 10.4269/ajtmh.1988.39.232.
Most adults in highly malarious areas have antibodies to the repeat region of the circumsporozoite protein of Plasmodium falciparum. To determine if a T cell epitope on the repeat region stimulated T cell help for this antibody, we used R32tet32, a recombinant construct derived from the repeat region of the circumsporozoite protein of P. falciparum, to stimulate in vitro mononuclear cells from residents of an area hyperendemic for malaria. Three groups differing in the length of time they had resided in a malarious area were studied. The percentage of individuals in each group who had positive antibody responses to R32tet32 increased with increased exposure to malaria. However, antibody positivity was not correlated with in vitro lymphocyte proliferation responses to the antigen. Lymphocytes from 79% of the individuals showing serum antibodies to R32tet32 failed to respond in a lymphocyte transformation assay, suggesting that T cell helper activity in these individuals was based upon the recognition of a T cell epitope not located within this peptide.
在疟疾高度流行地区，大多数成年人对恶性疟原虫环子孢子蛋白的重复区域具有抗体。为了确定重复区域上的T细胞表位是否刺激了针对该抗体的T细胞辅助作用，我们使用了R32tet32（一种源自恶性疟原虫环子孢子蛋白重复区域的重组构建体）来体外刺激来自疟疾高度流行地区居民的单核细胞。研究了在疟疾流行地区居住时间不同的三组人群。每组中对R32tet32产生阳性抗体反应的个体百分比随着疟疾暴露增加而升高。然而，抗体阳性与体外淋巴细胞对抗原的增殖反应无关。在淋巴细胞转化试验中，79%的血清中对R32tet32呈抗体阳性的个体的淋巴细胞无反应，这表明这些个体中的T细胞辅助活性是基于对该肽段以外的T细胞表位的识别。
