Brown A E, Webster H K, Pavanand K, Permpanich B, Sookto P, Sattabongkot J, Gingrich J B
Departments of Immunology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Trans R Soc Trop Med Hyg. 1989 Mar-Apr;83(2):154-7. doi: 10.1016/0035-9203(89)90623-8.
Most acute falciparum malaria patients mount an antibody response to the circumsporozoite (CS) protein which contains a dominant B-cell epitope. In order to investigate whether antibodies against other epitopes on the sporozoite surface may be important during a particular phase of infection or convalescence, we longitudinally studied the antibody responses of 13 Thai patients with acute falciparum malaria. Antibody comparisons were made using intact Plasmodium falciparum sporozoites in an indirect fluorescent antibody test and the recombinant peptide, R32tet32, as capture antigen in an enzyme-linked immunosorbent assay. Antibody response curves derived using the 2 methods were similar, and adsorption with R32tet32 greatly (greater than 95%) diminished anti-sporozoite activity in sera. Thus, peptide constructs containing the CS repeat region epitope, (NANP)n, can be used with confidence to assay anti-sporozoite antibodies, independent of both the time of infection and prior malaria history.
大多数急性恶性疟患者会对含有主要B细胞表位的环子孢子（CS）蛋白产生抗体反应。为了研究针对子孢子表面其他表位的抗体在感染或康复的特定阶段是否重要，我们对13例急性恶性疟泰国患者的抗体反应进行了纵向研究。在间接荧光抗体试验中使用完整的恶性疟原虫子孢子，并在酶联免疫吸附试验中使用重组肽R32tet32作为捕获抗原进行抗体比较。用这两种方法得出的抗体反应曲线相似，并且用R32tet32吸附可大大（超过95%）降低血清中的抗子孢子活性。因此，含有CS重复区域表位（NANP）n的肽构建体可放心用于检测抗子孢子抗体，而与感染时间和既往疟疾史无关。
