Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA.
J Virol. 2014 May;88(10):5877-80. doi: 10.1128/JVI.00476-14. Epub 2014 Mar 5.
Varicella-zoster virus (VZV) infection causes varicella, after which the virus becomes latent in ganglionic neurons. In tissue culture, VZV-infected human neurons remain viable at 2 weeks, whereas fibroblasts develop cytopathology. Next-generation RNA sequencing was used to compare VZV transcriptomes in neurons and fibroblasts and identified only 12 differentially transcribed genes of the 70 annotated VZV open reading frames (ORFs), suggesting that defective virus transcription does not account for the lack of cell death in VZV-infected neurons in vitro.
水痘带状疱疹病毒（VZV）感染会导致水痘，之后病毒潜伏在神经节神经元中。在组织培养中，VZV 感染的人类神经元在 2 周内仍然存活，而成纤维细胞则会出现细胞病变。下一代 RNA 测序用于比较神经元和成纤维细胞中的 VZV 转录组，结果仅在 70 个注释的 VZV 开放阅读框（ORF）中鉴定出 12 个差异转录基因，这表明体外 VZV 感染神经元中缺乏细胞死亡并不是由于病毒转录缺陷所致。
