死亡后不久获得的人三叉神经节中受限的水痘带状疱疹病毒转录。
Restricted varicella-zoster virus transcription in human trigeminal ganglia obtained soon after death.
机构信息
Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.
出版信息
J Virol. 2012 Sep;86(18):10203-6. doi: 10.1128/JVI.01331-12. Epub 2012 Jun 27.
Abstract
We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.
摘要
我们分析了 43 例潜伏性感染人类三叉神经节（TG）的水痘带状疱疹病毒（VZV）转录组，死后间隔时间（PMI）从 3.7 小时到 24 小时不等。多重逆转录酶 PCR（RT-PCR）显示，PMI<9 小时的 VZV 转录本。实时 PCR 表明 VZV ORF63 转录本水平显著增加（P=0.02），但随着 PMI 的延长，病毒 DNA 负担并没有增加。总的来说，随着 PMI 的延长，人类尸体 TG 中的 VZV 转录组的广度和 VZV ORF63 转录本水平均增加。
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