Area of Neuroscience, International School for Advanced Studies (SISSA) Trieste, Italy ; RIKEN Yokohama Institute, Center for Life Science Technologies, Division of Genomic Technologies Tsurumi-ku, Yokohama, Japan.
Area of Neuroscience, International School for Advanced Studies (SISSA) Trieste, Italy ; Cluster in Biomedicine (CBM), AREA Science Park Trieste, Italy.
Front Cell Neurosci. 2014 Feb 18;8:41. doi: 10.3389/fncel.2014.00041. eCollection 2014.
By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture. Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing and positioning transcribed LINEs as candidate regulatory RNAs for VRs expression.
通过将激光捕获显微切割与 nanoCAGE 技术和下一代测序相结合，我们已经鉴定出了在小鼠主要嗅觉上皮（MOE）中活跃启动子的全基因组集合。先前已经对大多数嗅觉受体（ORs）的转录起始位点（TSS）进行了定位，这增加了我们对其启动子结构的理解。在这里，我们表明，在我们对小鼠 MOE 的 nanoCAGE 文库中，我们检测到大量标签映射在包含 1 型和 2 型犁鼻器受体基因（V1Rs 和 V2Rs）的基因座中。这些基因座也表现出大量长散布核元件（LINEs）的表达。我们已经通过原位杂交、RT-PCR 和 qRT-PCR 验证了通过 nanoCAGE 检测到的选定受体的表达。这项工作扩展了能够在 MOE 中感知化学信号的受体的种类，表明 MOE 和 VNO 之间在信息素处理方面存在有趣的相互作用，并将转录的 LINEs 定位为 VRs 表达的候选调控 RNA。
