Agalliu Ilir, Xue Xiaonan, Cushman Mary, Cornell Elaine, Hsing Ann W, Kaplan Robert C, Anastos Kathryn, Rajpathak Swapnil, Ho Gloria Y F
Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, NY, USA.
Department of Medicine, University of Vermont, Colchester, VT, USA.
Results Immunol. 2013 Aug 5;3:79-84. doi: 10.1016/j.rinim.2013.07.001. eCollection 2013.
Multiplex assays are available to measure an array of circulating chemokines, soluble cytokine receptors and growth factors. However, there is limited information regarding whether these analytes are suitable for large-scale epidemiological studies to assess their relationships with chronic diseases, including cancer.
We examined detectability, assay repeatability, and 3-year within-subject reproducibility of plasma levels of 25 chemokines and 11 soluble receptors of cytokines and growth factors selected from the Human Millipore Panels. Plasma samples were obtained from 36 men (average age 62 years) and 17 women (average age 32 years) who participated in two epidemiological studies. Inter-assay and within-subject reproducibility were assessed by intraclass correlation coefficients (ICC).
All analytes, except lymphotactin (47% detectability), were detectable in >90% of plasma samples. Inter-assay reproducibility for all analytes in 36 men tested three times on separate days were good to excellent (ICCs: 0.71-1.00). Within-subject reproducibility in 17 women sampled three times in three years were excellent (ICC ≥ 0.75) for five chemokines (eotaxin, fractalkine, 6Ckine, eotaxin 3, and SDF-1α+β) and three soluble receptors (sIL-1R2, sIL-4R and sVEGFR2); ICCs were fair to good (0.4 ≤ ICC < 0.75) for 15 chemokines and eight soluble receptors. However, five chemokines (GRO, IP-10, MIP-1β, BCA-1, and MIP-3α) had ICC < 0.4, suggesting biological variability.
Multiplex assays for plasma levels of selected chemokines and soluble receptors showed good to excellent assay detectability and repeatability. Most analytes also had good 3-year within-subject reproducibility, indicating that a single measurement of these analytes may be used to assess biomarker-disease associations.
多重检测可用于测量一系列循环趋化因子、可溶性细胞因子受体和生长因子。然而，关于这些分析物是否适用于大规模流行病学研究以评估它们与包括癌症在内的慢性疾病之间的关系，相关信息有限。
我们检测了从人类密理博检测板中选出的25种趋化因子以及11种细胞因子和生长因子的可溶性受体的血浆水平的可检测性、检测重复性以及3年内个体内的再现性。血浆样本取自参与两项流行病学研究的36名男性（平均年龄62岁）和17名女性（平均年龄32岁）。通过组内相关系数（ICC）评估检测间和个体内的再现性。
除淋巴细胞趋化因子（可检测性为47%）外，所有分析物在超过90%的血浆样本中均可检测到。在不同日期对36名男性进行三次检测时，所有分析物的检测间再现性良好至优秀（ICC：0.71 - 1.00）。在三年内对17名女性进行三次采样时，五种趋化因子（嗜酸性粒细胞趋化因子、fractalkine、6Ckine、嗜酸性粒细胞趋化因子3和SDF - 1α + β）和三种可溶性受体（sIL - 1R2、sIL - 4R和sVEGFR2）的个体内再现性优秀（ICC≥0.75）；15种趋化因子和8种可溶性受体的ICC为中等至良好（0.4≤ICC＜0.75）。然而，五种趋化因子（GRO、IP - 10、MIP - 1β、BCA - 1和MIP - 3α）的ICC＜0.4，表明存在生物学变异性。
针对选定趋化因子和可溶性受体血浆水平的多重检测显示出良好至优秀的检测可检测性和重复性。大多数分析物在3年内个体内也具有良好的再现性，这表明对这些分析物进行单次测量可用于评估生物标志物与疾病的关联。
