Medeiros-Silva Daniela Carla, Dos Santos Moreira-Silva Eduardo Augusto, Assis Silva Gomes Juliana de, da Fonseca Flávio Guimarães, Correa-Oliveira Rodrigo
Centro de Pesquisas René Rachou (CPqRR), Fundação Oswaldo Cruz (Fiocruz), Ministério da Saúde, Avenida Augusto de Lima, 1715 - Barro Preto - CEP: 30190-002 - Belo Horizonte, MG - Brazil. ; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Brazil.
Departamento de Morfologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Brazil.
Results Immunol. 2013 Nov 9;3:104-13. doi: 10.1016/j.rinim.2013.10.002. eCollection 2013.
The present study evaluates the immune response of memory CD4(+) and CD8(+) T cells from patients following a natural Vaccinia virus (VACV) infection. A total of 42 individuals were involved in the study being: 22 previously infected individuals (vaccinated or not against smallpox) and 20 non-infected individuals (vaccinated or not). A short-term in vitro stimulation with UV-inactivated VACV of whole blood cells was performed. Our study showed that previously infected individuals have a lower percentage of CD4(+) T cells expressing lymph-node homing receptors (CD4(+)CD62L(+)CCR7(+)) and higher percentage of memory CD4(+) T cells subsets (CD4(+)CD45RO(High)) when compared with non-infected subjects, after in vitro viral stimulation. We also showed that infected individuals presented higher percentages of CD4(+) and CD8(+) memory T lymphocytes expressing IFN-γ when compared to non-infected individuals. We verified that the percentage of CD4(+) and CD8(+) T memory cells expressing TNF-α was higher in infected and non-infected vaccinated subjects when compared with non-infected unvaccinated individual. We also observed that previously infected individuals have higher percentages of CD8(+) T cells expressing lymph-node homing receptors (CCR7(+) and CD62L(+)) and that the memory T cells expressing IFN-γ and TNF-α were at higher percentages in the whole blood cells from infected and non-infected vaccinated individuals, when compared to unvaccinated non-infected subjects. Thus, our findings suggest that CD4(+) and CD8(+) T cells are involved in the immune memory response against Vaccinia virus natural infection.
本研究评估了天然痘苗病毒（VACV）感染后患者记忆性CD4(+)和CD8(+) T细胞的免疫反应。共有42人参与了该研究，其中22人为先前感染个体（接种过或未接种过天花疫苗），20人为未感染个体（接种过或未接种过疫苗）。对全血细胞进行了紫外线灭活VACV的短期体外刺激。我们的研究表明，与未感染个体相比，在体外病毒刺激后，先前感染个体表达淋巴结归巢受体的CD4(+) T细胞（CD4(+)CD62L(+)CCR7(+)）百分比更低，而记忆性CD4(+) T细胞亚群（CD4(+)CD45RO(High)）百分比更高。我们还表明，与未感染个体相比，感染个体中表达IFN-γ的CD4(+)和CD8(+)记忆性T淋巴细胞百分比更高。我们证实，与未接种疫苗的未感染个体相比，感染和未感染的接种疫苗个体中表达TNF-α的CD4(+)和CD8(+) T记忆细胞百分比更高。我们还观察到，先前感染个体中表达淋巴结归巢受体（CCR7(+)和CD62L(+)）的CD8(+) T细胞百分比更高，并且与未接种疫苗的未感染个体相比，感染和未感染的接种疫苗个体全血细胞中表达IFN-γ和TNF-α的记忆性T细胞百分比更高。因此，我们的研究结果表明，CD4(+)和CD8(+) T细胞参与了针对痘苗病毒天然感染的免疫记忆反应。
