China University of Geosciences, Lumo Road, Wuhan 430074, People's Republic of China.
School of Geophysics and Oil Resources, Yangtze University, Xueyuan Road, Jingzhou 434023, People's Republic of China.
Environ Technol. 2014 Jan-Feb;35(1-4):391-9. doi: 10.1080/09593330.2013.829504.
This paper presents a study of the effect of a combined biostimulation-bioaugmentation treatment applied to a clay-loam soil contaminated with 16,300 mg/kg of total petroleum hydrocarbons (TPH), which comprised 51% saturated hydrocarbons and 31% aromatic hydrocarbons. The bioaugmentation was performed with yeast Candida tropicalis SK21 isolated from petroleum-contaminated soil. The strain was able to grow in a pH range of 3-9 in liquid culture, and the optimum pH was found to be 6 for both growth and biosurfactant production. At pH 6, 96% and 42% of TPH were degraded by the strain at the initial diesel oil concentrations of 0.5% and 5% (v/v), respectively. The remediation via inoculating the yeast removed 83% of TPH in 180 days while the experiment with the indigenous microorganisms alone removed 61%. Microbial enumeration showed that the yeast SK21 could grow good in the soil. It was also found that dehydrogenase and polyphenoloxidase activities in soil were remarkably enhanced by the inoculation of the yeast.
本文研究了生物刺激-生物增强联合处理对受总石油烃（TPH）污染的粉质壤土的影响，该土壤中 TPH 含量为 16300mg/kg，其中饱和烃占 51%，芳烃占 31%。生物增强采用从石油污染土壤中分离出的热带假丝酵母 Candida tropicalis SK21 进行。该菌株能够在 pH 值为 3-9 的液体培养基中生长，最适 pH 值为 6，生长和生物表面活性剂的产生均在此 pH 值下达到最佳。在 pH 值为 6 时，该菌株在初始柴油浓度为 0.5%（v/v）和 5%（v/v）时分别降解了 96%和 42%的 TPH。通过接种酵母进行修复，在 180 天内去除了 83%的 TPH，而单独使用土著微生物的实验仅去除了 61%。微生物计数表明，酵母 SK21 在土壤中生长良好。还发现，接种酵母显著增强了土壤中脱氢酶和多酚氧化酶的活性。
