Schettini G, Meucci O, Florio T, Scala G, Landolfi E, Grimaldi M
Dept. of Pharmacology, II School of Medicine, Napoli, Italy.
Biochem Biophys Res Commun. 1988 Sep 30;155(3):1097-104. doi: 10.1016/s0006-291x(88)81253-1.
In the present study we investigated the effect of the interleukin 1 beta on intracellular free calcium concentrations in 235-1 cell line both in basal conditions and after stimulation by the calcium channel activator maitotoxin. Interleukin 1 beta (from 0.01 pM to 10 nM) was unable to significantly affect basal cytosolic free calcium levels in acute conditions. The preincubation of these cells with interleukin 1 beta for 48h modulates maitotoxin stimulation of calcium fluxes without modifying basal intracellular free calcium levels. Low concentrations of interleukin 1 beta (0.01 pM, 1 pM) caused a marked reduction of intracellular free calcium concentrations increase induced by maitotoxin while higher doses of the monokine potentiated maitotoxin stimulation of calcium fluxes. The specificity of interleukin 1 beta effect was tested by means of polyclonal anti-interleukin 1 beta antibody (titer 1:100) which significantly abolished the inhibitory effect of interleukin 1 beta on free cytosolic calcium levels. These results show that a long lasting interaction of interleukin 1 beta with its receptor is able to influence voltage-sensitive calcium channels activation induced by maitotoxin in 235-1 cells.
在本研究中，我们研究了白细胞介素1β在基础条件下以及钙通道激活剂刺尾鱼毒素刺激后对235 - 1细胞系细胞内游离钙浓度的影响。白细胞介素1β（浓度从0.01皮摩尔至10纳摩尔）在急性条件下无法显著影响基础胞质游离钙水平。将这些细胞与白细胞介素1β预孵育48小时可调节刺尾鱼毒素对钙通量的刺激，而不改变基础细胞内游离钙水平。低浓度的白细胞介素1β（0.01皮摩尔、1皮摩尔）可显著降低刺尾鱼毒素诱导的细胞内游离钙浓度升高，而较高剂量的该单核因子则增强刺尾鱼毒素对钙通量的刺激。通过多克隆抗白细胞介素1β抗体（效价1:100）测试了白细胞介素1β作用的特异性，该抗体显著消除了白细胞介素1β对胞质游离钙水平的抑制作用。这些结果表明，白细胞介素1β与其受体的长期相互作用能够影响刺尾鱼毒素在235 - 1细胞中诱导的电压敏感性钙通道激活。
