Takahashi M, Ohizumi Y, Yasumoto T
J Biol Chem. 1982 Jul 10;257(13):7287-89.
Effects of maitotoxin, the most potent marine toxin, were studied using a rat pheochromocytoma cell line, PC12h. A low concentration (10(-8) g/ml) of maitotoxin induced a profound increase in CA2+ influx into PC12h cells and the Ca2+-dependent release of [3H]norepinephrine from them. The effects of maitotoxin were not affected by treatment with tetrodotoxin (10(-6) M) and were observed even in the absence of external Na+. Furthermore, these effects were markedly inhibited or abolished by treatment with verapamil (30-300 microM), Mn2+ (5 mM), or tetracaine (1 mM). These results suggest that maitotoxin activates the voltage-dependent calcium channels of PC12h cells.
使用大鼠嗜铬细胞瘤细胞系PC12h研究了最具毒性的海洋毒素——刺尾鱼毒素的作用。低浓度（10^(-8) g/ml）的刺尾鱼毒素可显著增加Ca2+流入PC12h细胞，并使其Ca2+依赖性释放[3H]去甲肾上腺素。刺尾鱼毒素的作用不受河豚毒素（10^(-6) M）处理的影响，甚至在无细胞外Na+的情况下也可观察到。此外，维拉帕米（30 - 300 microM）、Mn2+（5 mM）或丁卡因（1 mM）处理可显著抑制或消除这些作用。这些结果表明，刺尾鱼毒素可激活PC12h细胞的电压依赖性钙通道。
