日本脑炎病毒潜伏感染中的免疫记忆。
Immunological memory in latent Japanese encephalitis virus infection.
作者信息
Kulshreshtha R, Mathur A, Chaturvedi U C
机构信息
Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
出版信息
Br J Exp Pathol. 1988 Aug;69(4):465-71.
Long term B-cell memory to Japanese encephalitis virus (JEV) in latently infected mice was investigated by adoptive cell transfer. Both IgM and IgG memory were elicited by antigen challenge or cyclophosphamide induced reactivation of virus. A weak antigen-specific IgM response for a brief period and a strong IgG response were detected in Swiss albino mice exposed to secondary infection. A correlation between the secondary IgM antibody and protection against JEV challenge was observed in adoptive transfer experiments. This was abrogated by pretreatment of the serum with 2-mercaptoethanol. Similarly secondary immune splenic T-cells up to day 5 post-reactivation provided protection. These results suggest that a long term antigen-specific IgM and IgG memory was induced by JEV challenge in latently infected mice. Further, the role of IgM antibody and T-cells in the response of mice to secondary JEV infection has been shown.
通过过继性细胞转移研究了潜伏感染小鼠对日本脑炎病毒（JEV）的长期B细胞记忆。抗原刺激或环磷酰胺诱导的病毒再激活均可引发IgM和IgG记忆。在遭受二次感染的瑞士白化小鼠中，检测到短期内微弱的抗原特异性IgM反应和强烈的IgG反应。在过继转移实验中观察到二次IgM抗体与抵抗JEV攻击的保护作用之间存在相关性。用2-巯基乙醇预处理血清可消除这种相关性。同样，再激活后直至第5天的二次免疫脾T细胞也提供了保护作用。这些结果表明，JEV攻击可在潜伏感染的小鼠中诱导长期的抗原特异性IgM和IgG记忆。此外，还显示了IgM抗体和T细胞在小鼠对JEV二次感染反应中的作用。
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